Luminescent conjugated oligothiophenes for sensitive fluorescent assignment of protein inclusion bodies.
Luminescent conjugated oligothiophenes for sensitive fluorescent assignment of protein inclusion bodies.
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DOI：
10.1002/cbic.201200731
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发表时间：
2013-03-18
期刊：
影响因子：
3.2
通讯作者：
Nilsson, K. Peter R.
中科院分区：
文献类型：
--
作者：
Klingstedt, Therse;Blechschmidt, Cristiane;Nogalska, Anna;Prokop, Stefan;Haggqvist, Bo;Danielsson, Olof;Engel, W. King;Askanas, Valerie;Heppner, Frank L.;Nilsson, K. Peter R.
关键词：
识别细胞内蛋白质沉积物的小疏水配体引起了极大的兴趣，因为蛋白质包涵体是几种退行性疾病的病理标志。在这里，我们报告，荧光淀粉样蛋白配体，称为发光共轭寡聚噻吩（LCOs），快速和高灵敏度检测蛋白包涵体在骨骼肌组织中的患者散发性包涵体肌炎（s-IBM）。具有至少五个噻吩单元的缀合骨架的LCO在结合时发射强荧光，并且显示与据报道在s-IBM蛋白包涵体中积累的蛋白质共定位。与传统的淀粉样蛋白配体相比，LCOs鉴定了更大比例的免疫阳性包涵体。当共轭噻吩骨架与末端羧基基团延伸时，LCO揭示了不同蛋白质包涵体之间的显著光谱差异。我们得出结论：1）LCO是鉴定蛋白包涵体的灵敏、快速和有力的工具，2）LCO比传统的淀粉样蛋白配体鉴定更广泛的蛋白包涵体。
Small hydrophobic ligands identifying intracellular protein deposits are of great interest, as protein inclusion bodies are the pathological hallmark of several degenerative diseases. Here we report that fluorescent amyloid ligands, termed luminescent conjugated oligothiophenes (LCOs), rapidly and with high sensitivity detect protein inclusion bodies in skeletal muscle tissue from patients with sporadic inclusion body myositis (s-IBM). LCOs having a conjugated backbone of at least five thiophene units emitted strong fluorescence upon binding, and showed co-localization with proteins reported to accumulate in s-IBM protein inclusion bodies. Compared with conventional amyloid ligands, LCOs identified a larger fraction of immunopositive inclusion bodies. When the conjugated thiophene backbone was extended with terminal carboxyl groups, the LCO revealed striking spectral differences between distinct protein inclusion bodies. We conclude that 1) LCOs are sensitive, rapid and powerful tools for identifying protein inclusion bodies and 2) LCOs identify a wider range of protein inclusion bodies than conventional amyloid ligands.
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影响因子：
3.2
作者：
Nilsson, K. Peter R.;Hammarstrom, Per;Inganas, Olle
通讯作者：
Inganas, Olle
影响因子：
2.9
作者：
Nilsson, KPR;Herland, A;Inganäs, O
通讯作者：
Inganäs, O
DOI：
10.1126/science.1173155
发表时间：
2009-07-17
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Maji SK;Perrin MH;Sawaya MR;Jessberger S;Vadodaria K;Rissman RA;Singru PS;Nilsson KP;Simon R;Schubert D;Eisenberg D;Rivier J;Sawchenko P;Vale W;Riek R
通讯作者：
Riek R
影响因子：
3.2
作者：
Klingstedt T;Aslund A;Simon RA;Johansson LB;Mason JJ;Nyström S;Hammarström P;Nilsson KP
通讯作者：
Nilsson KP
影响因子：
5.3
作者：
Nilsson, K. Peter R.;Joshi-Barr, Shivanjali;Sigurdson, Christina J.
通讯作者：
Sigurdson, Christina J.