Fine mapping and candidate gene analysis of gynoecy trait in chieh-qua (Benincasa hispida Cogn. var. chieh-qua How).
Fine mapping and candidate gene analysis of gynoecy trait in chieh-qua (Benincasa hispida Cogn. var. chieh-qua How).
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DOI：
10.3389/fpls.2023.1158735
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发表时间：
2023
影响因子：
5.6
通讯作者：
Peng, Qingwu
中科院分区：
文献类型：
--
作者：
Wang, Min;Yang, Songguang;Liu, Wei;Cao, Zhenqiang;Chen, Lin;Liu, Wenrui;Xie, Dasen;Yan, Jinqiang;Jiang, Biao;Peng, Qingwu
雌雌交配证明了杂交品种的早期生产和更高的产量，并提高了杂交种子生产的效率。因此，雌核的利用有利于菊科植物的遗传育种。然而，迄今为止，对菊科植物中雌性相关基因的了解还很少。本研究利用雌蕊系‘A36’与雌雄同株系‘SX’杂交的F2群体进行遗传作图，发现雌雄同株是由单隐性基因调控的。我们将其精细定位到一个530kb的区域，该区域位于Chr.8上的标记Indel-3和KASP145的两侧，包含8个候选基因。其中一个候选基因Bhi08G000345编码网络蛋白4 (CqNET4)，包含一个非同义SNP，导致异亮氨酸（ATA； I）氨基酸替换为蛋氨酸（ATG； M）。CqNET4在雌花中显著表达，只有3个与乙烯合成相关的基因在A36和SX之间显著表达。“我们的研究结果支持CqNET4是最可能导致双性同心术的候选基因，这与报道的双性同心术基因不同。”
Gynoecy demonstrates an earlier production of hybrids and a higher yield and improves the efficiency of hybrid seed production. Therefore, the utilization of gynoecy is beneficial for the genetic breeding of chieh-qua. However, little knowledge of gynoecious-related genes in chieh-qua has been reported until now. Here, we used an F2 population from the cross between the gynoecious line ‘A36’ and the monoecious line ‘SX’ for genetic mapping and revealed that chieh-qua gynoecy was regulated by a single recessive gene. We fine-mapped it into a 530-kb region flanked by the markers Indel-3 and KASP145 on Chr.8, which harbors eight candidate genes. One of the candidate genes, Bhi08G000345, encoding networked protein 4 (CqNET4), contained a non-synonymous SNP resulting in the amino acid substitution of isoleucine (ATA; I) to methionine (ATG; M). CqNET4 was prominently expressed in the female flower, and only three genes related to ethylene synthesis were significantly expressed between ‘A36’ and ‘SX.’ The results presented here provide support for the CqNET4 as the most likely candidate gene for chieh-qua gynoecy, which differed from the reported gynoecious genes.
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影响因子：
5.1
作者：
Healey A;Furtado A;Cooper T;Henry RJ
通讯作者：
Henry RJ
黄瓜 (Cucumis sativus L.) 的雌花期不稳定性是由于拷贝数变异依赖性雌性 (F) 基因座的不等交叉所致
影响因子：
8.7
作者：
Li, Zheng;Han, Yonghua;Weng, Yiqun
通讯作者：
Weng, Yiqun
影响因子：
56.9
作者：
Boualem, Adnane;Troadec, Christelle;Bendahmane, Abdelhafid
通讯作者：
Bendahmane, Abdelhafid
影响因子：
5.8
作者：
Abuin, Jose M.;Pichel, Juan C.;Amigo, Jorge
通讯作者：
Amigo, Jorge
DOI：
10.1093/bioinformatics/btp698
发表时间：
2010-03-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Li H;Durbin R
通讯作者：
Durbin R