Characterization of Circular Plasmid Dimers inBorrelia burgdorferi
Characterization of Circular Plasmid Dimers inBorrelia burgdorferi
复制标题
伯氏疏螺旋体环状质粒二聚体的表征
作者：
K. Tilly;L. Lubke;P. Rosa
ABSTRACT We have inactivated the ospC, oppAIV, andguaB genes on the 26-kb circular plasmid of Borrelia burgdorferi (cp26) by allelic exchange. On several occasions following such transformations, the cp26 of transformants had an aberrant mobility through agarose gels. Characterization of these cp26 molecules showed that the plasmid had dimerized. These dimers were quite stable during either selective or nonselective passage. Subsequent transformations with dimer DNA supported the hypothesis that in B. burgdorferi, transforming cp26 DNA most likely does not displace the resident homologous plasmid but rather must recombine in order to donate sequences that it carries. These serendipitous findings provide a mechanism for obtaining heterozygous complemented control strains when mutant phenotypes are characterized.
影响因子：
10.5
作者：
Solomon, JM;Lazazzera, BA;Grossman, AD
通讯作者：
Grossman, AD
影响因子：
15.9
作者：
Barbour, A G
通讯作者：
Barbour, A G
DOI：
10.4269/ajtmh.1995.53.397
发表时间：
1995-10-01
影响因子：
3.3
作者：
DESILVA, AM;FIKRIG, E
通讯作者：
FIKRIG, E