Salt-induced stabilization of EIN3/EIL1 confers salinity tolerance by deterring ROS accumulation in Arabidopsis.
Salt-induced stabilization of EIN3/EIL1 confers salinity tolerance by deterring ROS accumulation in Arabidopsis.
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盐诱导的 EIN3/EIL1 稳定性通过阻止拟南芥中 ROS 积累而赋予盐度耐受性
DOI：
10.1371/journal.pgen.1004664
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发表时间：
2014-10
期刊：
影响因子：
4.5
通讯作者：
Guo H
中科院分区：
文献类型：
--
作者：
Peng J;Li Z;Wen X;Li W;Shi H;Yang L;Zhu H;Guo H
乙烯被认为是一种调节多种应激反应的应激激素。盐分胁迫是限制植物生长发育的最严重的非生物胁迫之一。但乙烯信号如何参与植物对盐胁迫的反应还知之甚少。在这里，我们表明，经乙烯处理的拟南芥植株对盐胁迫的耐受性增强。功能获得和功能丧失的研究表明，EIN3(乙烯不敏感3)和EIL1(EIN3-like 1)这两个乙烯激活的转录因子是提高耐盐性的必要条件和充分条件。高盐度通过促进EIN3/EIL1靶向F-box蛋白EBF1和Ebf2的蛋白酶体降解而诱导EIN3/EIL1蛋白的积累，这是EIN2不依赖于EIN2的方式。全基因组转录组分析确定了一系列参与盐胁迫反应的SIED(盐诱导和EIN3/EIL1依赖)基因，包括几个编码活性氧物种(ROS)清除剂的基因。我们对EIN3类EIL1类盐敏感突变体进行了遗传筛选，并鉴定了5个EIN3直接靶基因，其中包括一个未知基因SIED1(At5g22270)，该基因编码一个93个氨基酸的多肽，参与ROS的清除。我们还发现，EIN3的激活通过直接转录调节POD的表达来增加POD的活性。因此，乙烯处理或EIN3激活能够阻止过量的ROS积累，提高对盐胁迫的耐性。综上所述，我们的研究为乙烯信号增强植物耐盐性的分子作用提供了新的见解，并阐明了EIN3在盐胁迫响应中的转录网络。高盐度作为一种世界性的非生物胁迫，限制了根的水分吸收，损害了细胞生理，限制了农作物的产量。乙烯是一种主要的植物激素，调节植物的发育以应对包括高盐胁迫在内的不利环境。然而，乙烯信号如何发挥作用以及乙烯信号如何在盐胁迫下进行调控的分子机制仍有待探索。在这里，我们报告了高盐度以不依赖于EIN2的方式诱导EIN3/EIL1蛋白积累和EBF1/2蛋白降解。此外，激活的EIN3阻止了过量的ROS积累，提高了耐盐性。转录组分析和功能研究揭示了EIN3在盐胁迫反应中的基因网络。对114个SIED(盐诱导和EIN3/EIL1依赖)基因的功能研究发现了一个新的ROS清除和耐盐性调节因子。这种对乙烯/盐相互调控的新认识将使人们能够更好地操纵和改造EIN3及其下游SIED基因，以提高植物对盐胁迫的耐受性和适应能力，特别是在未来那些重要的经济作物上。
Ethylene has been regarded as a stress hormone to regulate myriad stress responses. Salinity stress is one of the most serious abiotic stresses limiting plant growth and development. But how ethylene signaling is involved in plant response to salt stress is poorly understood. Here we showed that Arabidopsis plants pretreated with ethylene exhibited enhanced tolerance to salt stress. Gain- and loss-of-function studies demonstrated that EIN3 (ETHYLENE INSENSITIVE 3) and EIL1 (EIN3-LIKE 1), two ethylene-activated transcription factors, are necessary and sufficient for the enhanced salt tolerance. High salinity induced the accumulation of EIN3/EIL1 proteins by promoting the proteasomal degradation of two EIN3/EIL1-targeting F-box proteins, EBF1 and EBF2, in an EIN2-independent manner. Whole-genome transcriptome analysis identified a list of SIED (Salt-Induced and EIN3/EIL1-Dependent) genes that participate in salt stress responses, including several genes encoding reactive oxygen species (ROS) scavengers. We performed a genetic screen for ein3 eil1-like salt-hypersensitive mutants and identified 5 EIN3 direct target genes including a previously unknown gene, SIED1 (At5g22270), which encodes a 93-amino acid polypeptide involved in ROS dismissal. We also found that activation of EIN3 increased peroxidase (POD) activity through the direct transcriptional regulation of PODs expression. Accordingly, ethylene pretreatment or EIN3 activation was able to preclude excess ROS accumulation and increased tolerance to salt stress. Taken together, our study provides new insights into the molecular action of ethylene signaling to enhance plant salt tolerance, and elucidates the transcriptional network of EIN3 in salt stress response. High salinity, as a world-wide abiotic stress, restricts root water uptake, damages cell physiology, and limits the productivity of agricultural crops. Ethylene is a major phytohormone that regulates plant development in response to adverse environments, including high salt stress. However, the molecular mechanisms of how ethylene signal exerts its effect and how ethylene signaling is modulated upon salt stress remain to be explored. Here, we report that high salinity induces EIN3/EIL1 protein accumulation and EBF1/2 protein degradation in an EIN2-independent manner. Moreover, the activated EIN3 deters excess ROS accumulation and increases salt tolerance. Transcriptome analysis and functional studies reveal an EIN3-directed gene network in salt stress response. Functional studies of 114 SIED (Salt-Induced and EIN3/EIL1-Dependent) genes identify a novel regulator of ROS dismissal and salt tolerance. This new understanding of ethylene/salt mutual regulation would allow a better manipulation and engineering of EIN3 and its downstream SIED genes to enhance plant tolerance and adaption to salt stress, particularly in those economically important crops in the future.
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影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
56.9
作者：
Apse, MP;Aharon, GS;Blumwald, E
通讯作者：
Blumwald, E
DOI：
10.1073/pnas.0401698101
发表时间：
2004-04-27
影响因子：
11.1
作者：
Gagne, JM;Smalle, J;Vierstra, RD
通讯作者：
Vierstra, RD
影响因子：
56.9
作者：
Alonso, JM;Hirayama, T;Ecker, JR
通讯作者：
Ecker, JR
影响因子：
56.9
作者：
ECKER, JR
通讯作者：
ECKER, JR