Impact of microRNA expression in human atrial tissue in patients with atrial fibrillation undergoing cardiac surgery.
Impact of microRNA expression in human atrial tissue in patients with atrial fibrillation undergoing cardiac surgery.
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DOI：
10.1371/journal.pone.0073397
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Sawa Y
中科院分区：
文献类型：
--
作者：
Nishi H;Sakaguchi T;Miyagawa S;Yoshikawa Y;Fukushima S;Saito S;Ueno T;Kuratani T;Sawa Y
尽管microRNA （miRNA）在犬房颤模型中调节房颤的发生和/或进展，但其在人类中的潜在机制尚不清楚。我们推测心房组织中某些miRNA与房颤有关，并评估心脏手术患者心房组织中miRNA表达的关系。29例心血管手术患者右心房组织分为3组[A：慢性房颤或迷宫失败组，n=6；B：迷宫成功，n=10；C：窦性心律（SR） n=13]。采用高密度微阵列和逆转录聚合酶链反应（RT-PCR）检测miRNA表达。马松三色染色检测纤维化。miRNA芯片分析显示，与SR组相比，AF中miRNA-21、miRNA-23b、miRNA-199b和miRNA-208b升高。RT-PCR显示，与SR组相比，AF中miRNA-21（1.9倍）和miRNA-208b（4.2倍）升高。miRNA-21的表达量从C组增加到A组（A: 2.1倍，B: 1.8倍，C: 1.0倍）。纤维化程度由C向A增加（A: 43.0±12.9%,B: 21.3±6.1%,C: 11.9±3.1%）。纤维化百分比与miRNA-21表达相关（r=0.508, p<0.05）。与健康志愿者相比，房颤患者血浆miRNA-21水平显著降低（p<0.05）。miRNA-21在人心房组织中的表达被发现与心房纤维化有关，并可能影响房颤的发生，表明其作为心脏手术管理的生物标志物的有效性。
Although microRNA (miRNA) regulates initiation and/or progression of atrial fibrillation (AF) in canine AF models, the underlying mechanism in humans remains unclear. We speculated that certain miRNAs in atrial tissue are related to AF, and evaluated the relationship of miRNA expression in human atrial tissue in cardiac surgery patients. Right atrial tissues from 29 patients undergoing cardiovascular surgery were divided into 3 groups [A: chronic AF or unsuccessful maze, n=6; B: successful maze, n=10; C: sinus rhythm (SR) n=13]. miRNA expression was determined using high density microarrays and with Reverse transcriptase-polymerase chain reaction (RT-PCR). Fibrosis was examined using Masson trichrome staining. miRNA microarray analysis showed elevated miRNA-21, miRNA-23b, miRNA-199b, and miRNA-208b in AF as compared to SR groups. RT-PCR showed elevated miRNA-21 (1.9-fold) and miRNA-208b (4.2-fold) in AF as compared to the SR groups. miRNA-21 expression increased from Group C to A (A: 2.1-fold, B: 1.8-fold, C: 1.0-fold). Fibrosis increased from C to A (A: 43.0±12.9%, B: 21.3±6.1%, C: 11.9±3.1%). Percent fibrosis and miRNA-21 expression were correlated (r=0.508, p<0.05). The plasma levels of miRNA-21 in AF patients was significantly decreased as compared to the healthy volunteers (p<0.05). The expression of miRNA-21 in human atrial tissue was found to be related to atrial fibrosis and might affect AF occurrence, indicating its usefulness as a biomarker for cardiac surgery management.
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影响因子：
3.7
作者：
Liu Z;Zhou C;Liu Y;Wang S;Ye P;Miao X;Xia J
通讯作者：
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DOI：
10.1016/j.jtcvs.2010.08.067
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