The expression levels of plasma micoRNAs in atrial fibrillation patients.
The expression levels of plasma micoRNAs in atrial fibrillation patients.
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DOI：
10.1371/journal.pone.0044906
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Xia J
中科院分区：
文献类型：
--
作者：
Liu Z;Zhou C;Liu Y;Wang S;Ye P;Miao X;Xia J
MicroRNA （miRNA）已在人类血液中被发现。越来越多的研究表明，mirna可能作为疾病的生物标志物。我们研究了循环miRNA作为房颤（AF）预测因子的潜力。在本项目的发现阶段，我们使用大规模平行特征测序（MPSS）对5名健康对照者、5名阵发性心房颤动（PAF）单独患者和5名持续性心房颤动（PersAF）单独患者的miRNA表达谱（miRNome）进行了深入分析。在每个PAF组、PersAF组或对照组中发现22个特异性mirna异常。四个候选microrna （miRNA-146a、miRNA-150、miRNA-19a和miRNA-375）符合我们的选择标准，并在90个血浆样本的独立队列中使用TaqMan miRNA定量逆转录聚合酶链反应（qRT-PCR）进行评估。我们发现，与对照组相比，PAF患者的miRNA-150水平降低了约17倍，PersAF患者的miRNA-150水平降低了约20倍（P< 0.0001）。采用Logistic回归分析评估miRNA-150表达水平降低（比值比[OR] 1.96, 95%可信区间[CI] 1.5 ~ 3.57， P<0.001）、年龄（比值比[OR] 1.1, 95% CI 1.36 ~ 2.73， P<0.001）和左房内径（比值比[OR] 1.5, 95% CI 1.36 ~ 1.8， P<0.001）。每个都与房颤独立相关。许多已确定的与房颤相关的靶基因是炎症反应系统的一部分。我们发现血浆CRP水平与血浆miRNA-150水平呈负相关。综上所述，我们首先发现房颤患者血浆miRNA-150水平明显低于健康人。循环miRNA-150降低与房颤显著相关。
MicroRNA (miRNA) has been found in human blood. It has been increasingly suggested that miRNAs may serve as biomarkers for diseases. We examined the potential of circulating miRNA to serve as predictors of atrial fibrillation (AF). During the discovery stage of this project, we used massively parallel signature sequencing (MPSS) to carry out an in-depth analysis of the miRNA expression profile (miRNome) in 5 healthy controls, 5 patients with paroxysmal atrial fibrillation (PAF) alone, and 5 patients with persistent atrial fibrillation (PersAF) alone. Twenty-two specific miRNAs were found to be dysregulated in each PAF group, PersAF group, or control group. Four candidate microRNAs (miRNA-146a, miRNA-150, miRNA-19a, and miRNA-375) met our selection criteria and were evaluated in an independent cohort of 90 plasma samples using TaqMan miRNA quantitative reverse transcriptase–polymerase chain reaction (qRT-PCR). We found miRNA-150 levels to be reduced by a factor of approximately 17 in PAF relative to controls and a factor of approximately 20 in PersAF relative to controls (P<.0001). Logistic regression analyses were carried out to evaluate the reduced miRNA-150 expression levels (odds ratio [OR] 1.96, 95% confidence interval [CI] 1.5 to 3.57, P<0.001), age (OR 1.1, 95% CI 1.36 to 2.73, P<0.001), and Left atrial diameter (LAD) (OR 1.5, 95% CI 1.36 to 1.8, P<0.001). Each was independently associated with AF. Much of the identified target genes related to AF were part of the inflammatory response system. We found that plasma levels of CRP were negatively correlated with the plasma levels of miRNA-150. In summary, we firstly found that plasma miRNA-150 levels in from AF patients were substantially lower than that from healthy people. Circulating reduced miRNA-150 was significantly associated with AF.
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