Isolation and sequence of a cDNA encoding porcine mitochondrial NADP-specific isocitrate dehydrogenase.
Isolation and sequence of a cDNA encoding porcine mitochondrial NADP-specific isocitrate dehydrogenase.
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编码猪线粒体 NADP 特异性异柠檬酸脱氢酶的 cDNA 的分离和序列。
DOI：
10.1021/bi00142a007
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发表时间：
1992
期刊：
影响因子：
2.9
通讯作者：
McAlister-Henn,L
中科院分区：
文献类型：
--
作者：
Haselbeck,RJ;Colman,RF;McAlister-Henn,L
Department of Biological Chemistry, School of Medicine, University of California, Irvine, California 92717, and Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716 Received February 21, 1992; Revised Manuscript Received April 20, 1992 abstract: The cDNA for porcine mitochondrial NADP-specific isocitratedehydrogenase was isolated from a Xgtl 1 library using polymerase chain reaction. Translation of the DNA sequence gave a 413-residueamino acid sequence and a calculated molecular weight of 46600 for the mature polypeptide. Previously determined peptide sequences for the amino terminus and for internal tryptic peptides were all contained within the translated sequence. The porcine protein was found to share 63% residue identity with yeast mitochondrial NADP-specific isocitrate dehydrogenase and to be immunoreactive with an antiserum against theyeast protein. Highly conserved regions include residues which have been implicated in substrate and cofactor binding in previousstudies of the porcine enzyme. The two eucaryotic enzymes exhibit only minimal homology with the NADP-dependent isocitrate dehydrogenase from Escherichia coli, with the exception of a striking conservation of residues implicated in formation of the metal-isocitrate site of the procaryotic enzyme.
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DOI：
10.1073/pnas.86.22.8635
发表时间：
1989-11-01
影响因子：
11.1
作者：
HURLEY, JH;THORSNESS, PE;STROUD, RM
通讯作者：
STROUD, RM
影响因子：
3.2
作者：
KEYS, DA;MCALISTERHENN, L
通讯作者：
MCALISTERHENN, L
影响因子：
2.9
作者：
BARNES, LD;KUEHN, GD;ATKINSON, DE
通讯作者：
ATKINSON, DE
影响因子：
14.9
作者：
RALEIGH, EA;MURRAY, NE;HANAHAN, D
通讯作者：
HANAHAN, D
猪心脏 NADP 依赖性异柠檬酸脱氢酶的亲和标记和光谱探针。
影响因子：
3.9
作者：
Colman,RF
通讯作者：
Colman,RF