Construction and Characterization of a Cellulolytic Consortium Enriched from the Hindgut of Holotrichia parallela Larvae.
Construction and Characterization of a Cellulolytic Consortium Enriched from the Hindgut of Holotrichia parallela Larvae.
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平行黑鳃金龟幼虫后肠富集的纤维素分解菌群的构建和表征
DOI：
10.3390/ijms17101646
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发表时间：
2016-09-30
影响因子：
5.6
通讯作者：
Ding J
中科院分区：
文献类型：
--
作者：
Sheng P;Huang J;Zhang Z;Wang D;Tian X;Ding J
微生物协同降解稻草是目前最有吸引力的燃料替代物，为生物质转化提供了基础。利用微生物菌群对木质纤维素进行生物降解，可以减少协同酶不完全、终产物抑制等问题，本研究采用静态连续传代培养的方法，从大黑鳃金龟（Holotrichia parallela）幼虫后肠中富集了一株纤维素降解菌群。培养3d后，稻草的降解率可达83.1%，具有较强的纤维素降解活性。多样性分析结果表明，在富集过程中，细菌多样性和丰富度下降，变形菌门和螺旋体门，梭菌纲、ε变形菌纲和β变形菌纲，弓形杆菌属、密螺旋体属、丛毛单胞菌属和梭菌属均得到富集。其中一些是众所周知的典型的纤维素分解和半纤维素分解微生物。我们的研究结果表明，本文确定的微生物聚生体是用于降解废弃木质纤维素生物质的潜在候选者，并进一步突出了幼虫的后肠作为广泛和特定的纤维素分解和半纤维素分解微生物的储库。
Degradation of rice straw by cooperative microbial activities is at present the most attractive alternative to fuels and provides a basis for biomass conversion. The use of microbial consortia in the biodegradation of lignocelluloses could reduce problems such as incomplete synergistic enzymes, end-product inhibition, and so on. In this study, a cellulolytic microbial consortium was enriched from the hindgut of Holotrichia parallela larvae via continuous subcultivation (20 subcultures in total) under static conditions. The degradation ratio for rice straw was about 83.1% after three days of cultivation, indicating its strong cellulolytic activity. The diversity analysis results showed that the bacterial diversity and richness decreased during the consortium enrichment process, and the consortium enrichment process could lead to a significant enrichment of phyla Proteobacteria and Spirochaetes, classes Clostridia, Epsilonproteobacteria, and Betaproteobacteria, and genera Arcobacter, Treponema, Comamonas, and Clostridium. Some of these are well known as typical cellulolytic and hemicellulolytic microorganisms. Our results revealed that the microbial consortium identified herein is a potential candidate for use in the degradation of waste lignocellulosic biomass and further highlights the hindgut of the larvae as a reservoir of extensive and specific cellulolytic and hemicellulolytic microbes.
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DOI：
10.1196/annals.1419.002
发表时间：
2008-01-01
期刊：
INCREDIBLE ANAEROBES: FROM PHYSIOLOGY TO GENOMICS TO FUELS
影响因子：
--
作者：
Doi, Roy H.
通讯作者：
Doi, Roy H.
影响因子：
2.9
作者：
Alam, MZ;Fakhru'l-Rai, A;Molla, AH
通讯作者：
Molla, AH
影响因子：
11.4
作者：
Oh, SE;Van Ginkel, S;Logan, BE
通讯作者：
Logan, BE
平行黑鳃金龟幼虫后肠微生物纤维素酶和半纤维素酶基因的高遗传多样性
影响因子：
5.6
作者：
Sheng P;Li Y;Marshall SD;Zhang H
通讯作者：
Zhang H
DOI：
10.1099/00207713-33-3-605
发表时间：
1983-01-01
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
影响因子：
--
作者：
MCCLUNG, CR;PATRIQUIN, DG;DAVIS, RE
通讯作者：
DAVIS, RE