Prevalence of Borrelia miyamotoi in Ixodes ticks in Europe and the United States.

Prevalence of Borrelia miyamotoi in Ixodes ticks in Europe and the United States.
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DOI:
10.3201/eid2010.131583
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发表时间:
2014-10
影响因子:
11.8
通讯作者:
Eshoo MW
Eshoo MW
中科院分区:
医学2区
文献类型:
--
作者:
Crowder CD;Carolan HE;Rounds MA;Honig V;Mothes B;Haag H;Nolte O;Luft BJ;Grubhoffer L;Ecker DJ;Schutzer SE;Eshoo MW

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在欧洲和美国,蜱虫感染宫本氏疏螺旋体的比率因地点而有很大差异。宫本氏疏螺旋体是一种由蜱虫传播的与回归热有关的螺旋体,最近已被证明是一种人类病原体。为了确定蜱虫在蜱虫中的流行程度,我们采用PCR和电喷雾电离质谱法对2754只蜱虫进行了多种蜱传病原体的检测。从2008年到2012年,蜱虫是从美国的加利福尼亚、纽约、康涅狄格州、宾夕法尼亚州和印第安纳州以及欧洲的德国和捷克共和国收集的。此外,还对一株来自日本的分离株进行了鉴定。我们发现了三种不同的基因型,一种在北美,一种在欧洲,一种在日本。在调查的26个地点中,有16个地点的蜱感染宫宫氏蜱,感染率高达15.4%。这些结果表明该病原体分布广泛,表明在蜱虫居住的地区对人类存在暴露风险。
Infection rates of Ixodes ticks with Borrelia miyamotoi in Europe and the United States vary greatly based upon location. Borrelia miyamotoi, a relapsing fever-related spirochete transmitted by Ixodes ticks, has been recently shown to be a human pathogen. To characterize the prevalence of this organism in questing Ixodes ticks, we tested 2,754 ticks for a variety of tickborne pathogens by PCR and electrospray-ionization mass spectrometry. Ticks were collected from California, New York, Connecticut, Pennsylvania, and Indiana in the United States and from Germany and the Czech Republic in Europe from 2008 through 2012. In addition, an isolate from Japan was characterized. We found 3 distinct genotypes, 1 for North America, 1 for Europe, and 1 for Japan. We found B. miyamotoi infection in ticks in 16 of the 26 sites surveyed, with infection prevalence as high as 15.4%. These results show the widespread distribution of the pathogen, indicating an exposure risk to humans in areas where Ixodes ticks reside.
DOI: 10.1603/me12038
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