Incorporation of Apigenin and tt-Farnesol into dental composites to modulate the Streptococcus mutans virulence.
Incorporation of Apigenin and tt-Farnesol into dental composites to modulate the Streptococcus mutans virulence.
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DOI：
10.1016/j.dental.2020.12.005
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发表时间：
2021-04
期刊：
影响因子：
--
通讯作者：
Ferracane JL
中科院分区：
文献类型：
--
作者：
André CB;Rosalen PL;Giannini M;Bueno-Silva B;Pfeifer CS;Ferracane JL
这项体外研究的目的是将两种抗龋剂芹菜素和 tt-金合欢醇掺入树脂复合材料和树脂水泥中，以降低牙齿修复体周围变形链球菌的毒力。单独或一起添加芹菜素（Api，5mM）和tt-金合欢醇（Far，5mM），并与氟化物（F）混合。变形链球菌生物膜在复合圆盘上生长，并对干重、细菌活力和多糖（碱溶性、细胞内和水溶性）进行定量。变形链球菌生物膜的 CLSM 图像是在水储存三年后获得的。还分析了添加物对理化性质和复合比色参数的影响。添加物不会影响细菌的活力。 Api单独使用、与Far联用或与Far与F联用均降低了细菌干重、碱溶性和胞内多糖。三年后，含有添加物的复合材料在生物膜顶部呈现出更大的 EPS 基质。获得转化程度的统计差异；然而，最大聚合速率和固化动力学并未受到添加的影响。水溶性多糖、弯曲强度和弹性模量没有观察到差异。 Api 增加了复合材料的黄度。
The aim of this in vitro study was to incorporate two anti-caries agents, Apigenin and tt-Farnesol, to resin composite and resin cement to reduce the virulence of Streptococcus mutans around dental restorations. Apigenin (Api, 5mM) and tt-Farnesol (Far, 5mM) were added alone, together, and combined with fluoride (F). Biofilm of S. mutans was grown on composite discs, and the dry-weight, bacterial viability, and the polysaccharides (alkali-soluble, intracellular and water-soluble) were quantified. CLSM images of the S. mutans biofilm were obtained after three years of water-storage. The effect of the additions on the physicochemical properties and the composite colorimetric parameters were also analyzed. The additions did not affect bacterial viability. Api alone and combined with Far or combined with Far and F decreased the bacterial dry-weight, alkali-soluble and intracellular polysaccharides. After three years, the composites containing the additions presented a greater EPS matrix on the top of biofilm. Statistical difference was obtained for the degree of conversion; however, the maximum polymerization rate and curing kinetics were unaffected by the additions. No difference was observed for the water-soluble polysaccharides, flexural strength, and elastic modulus. Api increased the yellowness of the composites.
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影响因子：
4.2
作者：
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