Evaluation of Ductal Tissue in Coarctation of the Aorta Using X-Ray Phase-Contrast Tomography
Evaluation of Ductal Tissue in Coarctation of the Aorta Using X-Ray Phase-Contrast Tomography
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使用 X 射线相差断层扫描评估主动脉缩窄的导管组织
DOI：
10.1007/s00246-020-02526-5
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发表时间：
2021
影响因子：
1.6
通讯作者：
Oshima Yoshihiro
中科院分区：
文献类型：
--
作者：
Iwaki Ryuma;Matsuhisa Hironori;Minamisawa Susumu;Akaike Toru;Hoshino Masato;Yagi Naoto;Morita Kiyozo;Shinohara Gen;Kaneko Yukihiro;Yoshitake Syuichi;Takahashi Masashi;Tsukube Takuro;Oshima Yoshihiro
我们评估了X线相位对比断层扫描（XPCT）的组织学准确性，并研究了主动脉缩窄（CoA）标本的三维（3D）导管组织分布。我们使用了9个CoA样本，包括主动脉峡部、动脉导管（DA）及其汇合处。使用XPCT获得3D图像。扫描后，使用弹性货车Gieson（EVG）染色和转录因子AP-2 β（TFAP 2 B）免疫染色对样本进行组织学评价。XPCT断层图像清晰显示导管组织分布为低密度区。与EVG染色比较，主动脉壁质量密度与弹性纤维形成呈正相关（R= 0.69，P < 0.001）。TFAP 2B表达与XPCT图像上的低密度区（包括内膜厚度）一致。在3D成像上，DA止点至导管中膜远端和导管侧内膜的距离分别为1.63 ± 0.22 mm和2.70 ± 0.55 mm。在短轴视图中，导管组织进入主动脉腔的后部延伸为降主动脉直径的79 ± 18%。在三个标本中，主动脉壁完全被导管组织占据。导管内膜比导管中膜向远端和外侧扩展。XPCT图像的对比度分辨率与组织学评估相当。基于3D图像，我们得出结论，需要完全切除内膜厚度，包括DA插入的对侧，以消除残留的导管组织并防止术后再缩窄。
We assessed the histological accuracy of X-ray phase-contrast tomography (XPCT) and investigated three-dimensional (3D) ductal tissue distribution in coarctation of the aorta (CoA) specimens. We used nine CoA samples, including the aortic isthmus, ductus arteriosus (DA), and their confluences. 3D images were obtained using XPCT. After scanning, the samples were histologically evaluated using elastica van Gieson (EVG) staining and transcription factor AP-2 beta (TFAP2B) immunostaining. XPCT sectional images clearly depicted ductal tissue distribution as low-density areas. In comparison with EVG staining, the mass density of the aortic wall positively correlated with elastic fiber formation (R= 0.69,P< 0.001). TFAP2B expression was consistent with low-density area including intimal thickness on XPCT images. On 3D imaging, the distances from the DA insertion to the distal terminal of the ductal media and to the intima on the ductal side were 1.63 ± 0.22 mm and 2.70 ± 0.55 mm, respectively. In the short-axis view, the posterior extension of the ductal tissue into the aortic lumen was 79 ± 18% of the diameter of the descending aorta. In three specimens, the aortic wall was entirely occupied by ductal tissue. The ductal intima spread more distally and laterally than the ductal media. The contrast resolution of XPCT images was comparable to that of histological assessment. Based on the 3D images, we conclude that complete resection of intimal thickness, including the opposite side of the DA insertion, is required to eliminate residual ductal tissue and to prevent postoperative re-coarctation.
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影响因子：
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作者：
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DOI：
10.1053/j.pcsu.2016.09.007
发表时间：
2017-01-01
期刊：
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影响因子：
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作者：
De Leon, Luis E;McKenzie, E Dean
通讯作者：
McKenzie, E Dean
缩窄球囊血管成形术后再狭窄：与动脉导管的关系
影响因子：
1.4
作者：
Ken Takahashi;T. Ino;M. Ohkubo;K. Akimoto;Masahiko Kishirou
通讯作者：
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