NHR-23 activity is necessary for C. elegans developmental progression and apical extracellular matrix structure and function.
NHR-23 activity is necessary for C. elegans developmental progression and apical extracellular matrix structure and function.
复制标题
DOI：
10.1242/dev.201085
复制
发表时间：
2023-05-15
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
Nematode molting is a remarkable process where animals must repeatedly build a new apical extracellular matrix (aECM) beneath a previously built aECM that is subsequently shed. The nuclear hormone receptor NHR-23 (also known as NR1F1) is an important regulator of C. elegans molting. NHR-23 expression oscillates in the epidermal epithelium, and soma-specific NHR-23 depletion causes severe developmental delay and death. Tissue-specific RNAi suggests that nhr-23 acts primarily in seam and hypodermal cells. NHR-23 coordinates the expression of factors involved in molting, lipid transport/metabolism and remodeling of the aECM. NHR-23 depletion causes dampened expression of a nas-37 promoter reporter and a loss of reporter oscillation. The cuticle collagen ROL-6 and zona pellucida protein NOAH-1 display aberrant annular localization and severe disorganization over the seam cells after NHR-23 depletion, while the expression of the adult-specific cuticle collagen BLI-1 is diminished and frequently found in patches. Consistent with these localization defects, the cuticle barrier is severely compromised when NHR-23 is depleted. Together, this work provides insight into how NHR-23 acts in the seam and hypodermal cells to coordinate aECM regeneration during development. Summary: Activity of NHR-23, a C. elegans transcription factor, is necessary in seam and hypodermal cells to promote molting and timely development.
登录
查看更多内容
DOI：
10.1093/g3journal/jkad032
发表时间：
2023-04-11
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
通讯作者：
--
秀丽隐杆线虫的角质层：其隔离和部分特征。
影响因子：
7.8
作者：
Cox, G N;Kusch, M;Edgar, R S
通讯作者：
Edgar, R S
影响因子：
--
作者：
Vo AA;Levenson MT;Ragle JM;Ward JD
通讯作者：
Ward JD
影响因子：
5.3
作者：
Campbell, Lioudmila A.;Faivre, Emily J.;Ingraham, Holly A.
通讯作者：
Ingraham, Holly A.
影响因子：
48
作者：
Gibson, Daniel G.;Young, Lei;Smith, Hamilton O.
通讯作者：
Smith, Hamilton O.