Telomerase immortalization of human corneal endothelial cells yields functional hexagonal monolayers.
Telomerase immortalization of human corneal endothelial cells yields functional hexagonal monolayers.
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DOI：
10.1371/journal.pone.0051427
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Jurkunas UV
中科院分区：
文献类型：
--
作者：
Schmedt T;Chen Y;Nguyen TT;Li S;Bonanno JA;Jurkunas UV
人角膜内皮细胞（HCEnCs）是一种单层六边形细胞，其主要功能是通过调节角膜水合作用来维持角膜清晰度。hccs来源于神经嵴，并在有丝分裂后被阻滞。因此，由于衰老或角膜内皮疾病导致的细胞损失导致角膜水肿和失明，这是角膜移植的主要指征。在这里，我们展示了分布在原代细胞中的形态不同的hccs亚群的存在，并表现出增强的自我更新能力和缺乏细胞衰老的表型迹象。这些均匀和六边形的HCEnCs （HCEnCs -21）的菌落被选择性地分离出来，并显示出高增殖潜力，这依赖于内源性端粒酶和细胞周期蛋白D/CDK4的上调。端粒酶进一步转导HCEnC-21产生了高度增殖的角膜内皮细胞系（hcenc - 21t），该细胞系没有致癌转化，并保留了关键的角膜内皮细胞特征和功能。该研究将对角膜细胞生物学和再生医学领域产生重大影响。
Human corneal endothelial cells (HCEnCs) form a monolayer of hexagonal cells whose main function is to maintain corneal clarity by regulating corneal hydration. HCEnCs are derived from neural crest and are arrested in the post-mitotic state. Thus cell loss due to aging or corneal endothelial disorders leads to corneal edema and blindness–the leading indication for corneal transplantation. Here we show the existence of morphologically distinct subpopulations of HCEnCs that are interspersed among primary cells and exhibit enhanced self-renewal competence and lack of phenotypic signs of cellular senescence. Colonies of these uniform and hexagonal HCEnCs (HCEnC-21) were selectively isolated and demonstrated high proliferative potential that was dependent on endogenous upregulation of telomerase and cyclin D/CDK4. Further transduction of HCEnC-21 with telomerase yielded a highly proliferative corneal endothelial cell line (HCEnT-21T) that was devoid of oncogenic transformation and retained critical corneal endothelial cell characteristics and functionality. This study will significantly impact the fields of corneal cell biology and regenerative medicine.
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影响因子：
3.7
作者：
HAYFLICK, L;MOORHEAD, PS
通讯作者：
MOORHEAD, PS
影响因子：
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作者：
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影响因子：
8
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
56.9
作者：
Bodnar, AG;Ouellette, M;Wright, WE
通讯作者：
Wright, WE