Genetic framework for flowering-time regulation by ambient temperature-responsive miRNAs in Arabidopsis.
Genetic framework for flowering-time regulation by ambient temperature-responsive miRNAs in Arabidopsis.
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DOI：
10.1093/nar/gkp1240
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发表时间：
2010-05
影响因子：
14.9
通讯作者：
Ahn JH
中科院分区：
文献类型：
--
作者：
Lee H;Yoo SJ;Lee JH;Kim W;Yoo SK;Fitzgerald H;Carrington JC;Ahn JH
开花是受环境温度变化影响的主要性状。植物microRNAs（miRNAs）是一类在植物生长发育过程中起重要调控作用的非编码小分子RNA。为了阐明小分子RNA调控拟南芥开花时间的机制，本研究通过miRNA微阵列和北方杂交技术，在拟南芥中鉴定了6个环境温度响应型miR156、miR163、miR169、miR172、miR398和miR399。我们还通过miRNA北方杂交分析确定了120个独特的miRNA基因座对环境温度变化的表达谱。在两种不同的温度（16和23°C）下，环境温度响应性miRNAs及其靶基因的表达在很大程度上与温度相关。有趣的是，在短营养期（SVP）的病变，在热敏通路内的关键调节器，引起miR172和其靶基因的子集的表达的改变，提供了热敏通路基因和miR172之间的联系。miR172过表达的植物表现出不依赖于温度的早花表型，表明miR172表达的调节导致温度不敏感。综上所述，我们的研究结果表明，在非胁迫温度条件下，环境温度响应性miRNA的开花时间调控的遗传框架。
Flowering is the primary trait affected by ambient temperature changes. Plant microRNAs (miRNAs) are small non-coding RNAs playing an important regulatory role in plant development. In this study, to elucidate the mechanism of flowering-time regulation by small RNAs, we identified six ambient temperature-responsive miRNAs (miR156, miR163, miR169, miR172, miR398 and miR399) in Arabidopsis via miRNA microarray and northern hybridization analyses. We also determined the expression profile of 120 unique miRNA loci in response to ambient temperature changes by miRNA northern hybridization analysis. The expression of the ambient temperature-responsive miRNAs and their target genes was largely anticorrelated at two different temperatures (16 and 23°C). Interestingly, a lesion in short vegetative phase (SVP), a key regulator within the thermosensory pathway, caused alteration in the expression of miR172 and a subset of its target genes, providing a link between a thermosensory pathway gene and miR172. The miR172-overexpressing plants showed a temperature-independent early flowering phenotype, suggesting that modulation of miR172 expression leads to temperature insensitivity. Taken together, our results suggest a genetic framework for flowering-time regulation by ambient temperature-responsive miRNAs under non-stress temperature conditions.
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影响因子：
3.7
作者：
Fahlgren N;Howell MD;Kasschau KD;Chapman EJ;Sullivan CM;Cumbie JS;Givan SA;Law TF;Grant SR;Dangl JL;Carrington JC
通讯作者：
Carrington JC
影响因子：
56.9
作者：
Kobayashi, Y;Kaya, H;Araki, T
通讯作者：
Araki, T
影响因子：
7.2
作者：
Jang, Seonghoe;Torti, Stefano;Coupland, George
通讯作者：
Coupland, George
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
16
作者：
Jones-Rhoades, MW;Bartel, DP
通讯作者：
Bartel, DP