Microfluidic Chip Device for In Situ Mixing and Fabrication of Hydrogel Microspheres via Michael-Type Addition.
Microfluidic Chip Device for In Situ Mixing and Fabrication of Hydrogel Microspheres via Michael-Type Addition.
复制标题
DOI：
10.1021/acs.langmuir.1c01739
复制
发表时间：
2021-10-12
期刊：
影响因子：
3.9
通讯作者：
Zustiak, Silviya P.
中科院分区：
文献类型：
--
作者：
Sheth, Saahil;Stealey, Samuel;Morgan, Nicole Y.;Zustiak, Silviya P.
水凝胶微球被寻求用于各种生物医学应用，包括治疗和细胞递送、传感器和润滑剂。具有均匀尺寸和性质的水凝胶微球的稳健制造可以使用依赖于液滴形成和随后的凝胶化以形成微球的微流体系统来实现。当在液滴形成之后引发凝胶化时，这样的系统工作良好，但是对于其中在液滴形成之前引发凝胶化的定时凝胶化系统是不实用的;过早的凝胶化可能导致装置堵塞、由于前体溶液中的粘度变化而导致的可变微球直径以及在单次运行中产生的有限数量的微球。为了能够从定时凝胶化水凝胶系统微流体制造微球，需要原位混合区域，使得可以单独添加各种水凝胶前体组分。在这里，我们设计并评估了三种混合装置在液滴形成和随后的凝胶化之前混合水凝胶前体溶液的有效性。蛇形几何形状被认为是最有效的，并进一步改善了包括一个支柱阵列，以增加搅拌。优化的装置被证明可以充分混合前体溶液，并能够制造单分散聚乙二醇微球，为定时凝胶化水凝胶系统提供了巨大的潜力。
Hydrogel microspheres are sought for a variety of biomedical applications, including therapeutic and cellular delivery, sensors, and lubricants. Robust fabrication of hydrogel microspheres with uniform sizes and properties can be achieved using microfluidic systems that rely on droplet formation and subsequent gelation to form microspheres. Such systems work well when gelation is initiated after droplet formation but are not practical for timed-gelation systems where gelation is initiated prior to droplet formation; premature gelation can lead to device blockage, variable microsphere diameter due to viscosity changes in the precursor solution, and limited numbers of microspheres produced in a single run. To enable microfluidic fabrication of microspheres from timed-gelation hydrogel systems, an in situ mixing region is needed, so that various hydrogel precursor components can be added separately. Here, we designed and evaluated three mixing devices for their effectiveness at mixing hydrogel precursor solutions prior to droplet formation and subsequent gelation. The serpentine geometry was found to be most effective and was further improved with the inclusion of a pillar array to increase agitation. The optimized device was shown to fully mix precursor solutions and enable the fabrication of monodisperse polyethylene glycol microspheres, offering great potential for use in timed gelation hydrogel systems.
登录
查看更多内容
影响因子：
3.4
作者：
Clark J;Kaufman M;Fodor PS
通讯作者：
Fodor PS
影响因子：
9.7
作者：
Franco, C. L.;Price, J.;West, J. L.
通讯作者：
West, J. L.
影响因子：
2.8
作者：
Chen, Lingxin;Wang, Guoqing;Song, Joon Myong
通讯作者：
Song, Joon Myong
影响因子：
4.1
作者：
Esfahani RR;Jun H;Rahmani S;Miller A;Lahann J
通讯作者：
Lahann J
影响因子：
2.8
作者：
Chung, C. K.;Shih, T. R.
通讯作者：
Shih, T. R.