Influence of the nitrification inhibitor DMPP on the community composition of ammonia-oxidizing bacteria at microsites with increasing distance from the fertilizer zone
Influence of the nitrification inhibitor DMPP on the community composition of ammonia-oxidizing bacteria at microsites with increasing distance from the fertilizer zone
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硝化抑制剂DMPP对距施肥区距离增加的微点氨氧化细菌群落组成的影响
DOI：
10.1007/s00374-012-0692-3
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发表时间：
2012-05
期刊：
影响因子：
--
通讯作者：
秦江涛
中科院分区：
文献类型：
--
作者：
秦江涛
在实验室条件下，采用微宇宙实验方法，研究了硝化抑制剂3，4-二甲基吡唑磷酸盐（DMPP）对厘米尺度下氮转化和氨氧化细菌（AOB）群落组成的影响。28天后，从用尿素或尿素和DMPP处理的土壤中收集样品，距离肥料区的距离增加;该距离在水平和垂直方向上的范围为0至5 cm。结果表明，DMPP处理显著提高了土壤pH值、NH 4 +-N和矿质氮（NH 4 +-N、NO3--N和NO2--N）含量，降低了（NO3-+ NO2-）-N含量，且这种效应随着距离施肥区的增加而减弱。变性梯度凝胶电泳的指纹图谱表明，由于尿素处理中NH 4 +-N浓度的降低，随着与施肥区距离的增加，条带数减少。与单施尿素相比，DMPP处理提高了土壤NH 4 +-N浓度，降低了0 ~ 3cm范围内氨氧化细菌的多样性，但提高了3 ~ 5cm范围内氨氧化细菌的多样性。系统发育分析表明，硝化螺菌类群3占优势。因此，硝化抑制剂DMPP通过增加与施肥区的距离来改变AOB群落的组成，这可能与土壤pH和无机氮浓度的变化有关。
The effect of the nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP) on N transformations and composition of ammonia-oxidizing bacteria (AOB) communities was investigated at the centimeter scale in a microcosm experiment under laboratory conditions. After 28 days, samples were collected from soil treated with urea or urea and DMPP at increasing distance from the fertilizer zone; this distance ranged from 0 to 5 cm in both horizontal and vertical directions. The results showed that DMPP application significantly increased soil pH and NH4+-N and mineral N (NH4+-N, NO3−-N, and NO2−-N) concentrations but decreased (NO3−+ NO2−)-N concentration, and such effect was decreased by increasing the distance from the fertilizer zone. Fingerprint profiles of denaturing gradient gel electrophoresis showed that the number of bands decreased by increasing the distance from the fertilizer zone due to decreasing NH4+-N concentrations in the urea treatment. Compared to urea applied alone, DMPP application increased NH4+-N concentrations and decreased AOB diversity from 0 to 3 cm but promoted diversity from 3 to 5 cm distance from the fertilizer zone. A phylogenetic analysis showed that AOB communities were dominated byNitrosospiracluster 3. Therefore, the nitrification inhibitor DMPP modified the composition of AOB communities by increasing the distance from the fertilizer zone and this probably was related to the changes in soil pH and inorganic N concentration.
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DOI：
10.1007/bf01054805
发表时间：
1990-02
期刊：
Fertilizer research
影响因子：
--
作者：
B. Zacherl;A. Amberger
通讯作者：
B. Zacherl;A. Amberger
影响因子：
5.1
作者：
G. Webster;T. Embley;Thomas E. Freitag;Zena Smith;J. Prosser
通讯作者：
G. Webster;T. Embley;Thomas E. Freitag;Zena Smith;J. Prosser
影响因子：
1.7
作者：
B. Zebarth;P. Milburn
通讯作者：
B. Zebarth;P. Milburn
影响因子：
9.7
作者：
Chen, Deli;Suter, Helen C.;Edis, Robert
通讯作者：
Edis, Robert
影响因子：
8.8
作者：
Kleineidam, Kristina;Kosmrlj, Kristina;Schloter, Michael
通讯作者：
Schloter, Michael