Identification and Characterization of piRNA-Like Small RNAs in the Gonad of Sea Urchin (Strongylocentrotus nudus)
Identification and Characterization of piRNA-Like Small RNAs in the Gonad of Sea Urchin (Strongylocentrotus nudus)
复制标题
海胆 (Strongylocentrotus nudus) 性腺中 piRNA 样小 RNA 的鉴定和表征
作者：
Zhenlin Wei;Xiaolin Liu;Huilin Zhang
Piwi相互作用RNAs(PiRNAs)及其伙伴PIWI蛋白在生育、生殖系干细胞发育以及动物基因组的基本控制和进化中发挥着重要作用。然而，关于海胆中的piRNA种群的研究很少，海胆是一种广泛用于发育和遗传学研究的模式动物。利用SolexA测序技术，我们鉴定了13,051个在裸眼球虫雄性性腺中表达的PiRNA样RNA。通过基因芯片分析，在202个检测的RNA中，94个序列在雌性性腺中表达，表明雄性和雌性性腺都是类似piRNA的RNA丰富的器官。这些RNA在5‘端有“U”或在10的位置有“A”，大小从26到30个核苷酸，主要是28nT的长度，并倾向于在基因组的小区域聚集，在支架78427中获得了最长的PiRNA样RNA富集区，约5.5kb。比对结果表明，有11个RNA与已知的piRNAs同源。此外，BLASTn检索海胆重复元件数据库，发现这些piRNA样RNA与101种DNA转座子和反转录转座子相匹配，其中SPRP1、Harbinger-N2、iggyBac-N10、SINE2-1和iggyBac-N11是最常见的命中元件，表明这些piRNA样RNA具有转座子沉默功能。
Piwi-interacting RNAs (piRNAs) and their partner PIWI proteins play an essential role in fertility, germline stem cell development, as well as the basic control and evolution of animal genomes. However, research was rare with regard to piRNA population in sea urchin, a model animal intensively used for development and genetics studies. Utilizing Solexa sequencing, we present an identification of 13,051 piRNA-like RNAs expressed in male gonad of Strongylocentrotus nudus. Out of 202 tested RNAs, 94 sequences were confirmed to express in female gonad using microarray assay, suggesting that both male and female gonads are piRNA-like RNA-enriched organs. These RNAs with “U” at the 5′ end or “A” at position of 10, in size from 26 to 30 nucleotides, were predominantly 28 nt in length and tend to be clustered in small regions in genome, achieving the longest piRNA-like RNA-enriched region about 5.5 kb in scaffold78427. Alignment results showed 11 RNAs were homologous to the known piRNAs. Furthermore, BLASTn searching against sea urchin repeat element database showed these piRNA-like RNAs matched to 101 types of DNA transposons and retrotransposons, of which SPRP1, Harbinger-N2, piggyBac-N10, SINE2-1, and piggyBac-N11 were the most frequent hit elements, suggesting a transposon silencing function of these piRNA-like RNAs.
登录
查看更多内容
DOI：
10.1073/pnas.0600833103
发表时间：
2006-03-21
影响因子：
11.1
作者：
Kapitonov, VV;Jurka, J
通讯作者：
Jurka, J
影响因子：
11.8
作者：
Deng, W;Lin, HF
通讯作者：
Lin, HF
影响因子：
7
作者：
Armisen, Javier;Gilchrist, Michael J.;Miska, Eric A.
通讯作者：
Miska, Eric A.
影响因子：
2.9
作者：
Gao, XL;Gulari, E;Zhou, XC
通讯作者：
Zhou, XC
DOI：
10.1146/annurev.cellbio.24.110707.175327
发表时间：
2009
影响因子：
11.3
作者：
Thomson T;Lin H
通讯作者：
Lin H