Subunit compositions of the RNA-silencing enzymes Pol IV and Pol V reveal their origins as specialized forms of RNA polymerase II.
Subunit compositions of the RNA-silencing enzymes Pol IV and Pol V reveal their origins as specialized forms of RNA polymerase II.
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DOI：
10.1016/j.molcel.2008.12.015
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发表时间：
2009-01-30
期刊：
影响因子：
16
通讯作者：
Pikaard, Craig S.
中科院分区：
文献类型：
--
作者：
Ream, Thomas S.;Haag, Jeremy R.;Wierzbicki, Andrzej T.;Nicora, Carrie D.;Norbeck, Angela D.;Zhu, Jian-Kang;Hagen, Gretchen;Guilfoyle, Thomas J.;Pasa-Tolic, Ljiljana;Pikaard, Craig S.
除了存在于所有真核生物中的基本RNA聚合酶--RNA聚合酶I、II和III之外，植物还有另外两种核RNA聚合酶，简称POL IV和POL V，它们在siRNA介导的DNA甲基化和基因沉默中发挥着非多余的作用。我们发现拟南芥POL IV和POL V由与POL II平行或相同的12个亚基组成。POL IV的4个亚基与它们的POL II平行亚基不同，POL V的6个亚基不同于它们的POL II平行亚基，以及POL IV和POL V之间的4个亚基不同。重要的是，亚基差异发生在相对于模板进入和RNA退出路径的关键位置。我们的发现支持Pol IV和Pol V是类似Pol II的酶的假设，它们在RNA沉默和基因组防御的非编码转录本的产生中进化出特殊的作用。
In addition to RNA polymerases I, II, and III, the essential RNA polymerases present in all eukaryotes, plants have two additional nuclear RNA polymerases, abbreviated as Pol IV and Pol V, that play nonredundant roles in siRNA-directed DNA methylation and gene silencing. We show that Arabidopsis Pol IV and Pol V are composed of subunits that are paralogous or identical to the 12 subunits of Pol II. Four subunits of Pol IV are distinct from their Pol II paralogs, six subunits of Pol V are distinct from their Pol II paralogs, and four subunits differ between Pol IV and Pol V. Importantly, the subunit differences occur in key positions relative to the template entry and RNA exit paths. Our findings support the hypothesis that Pol IV and Pol V are Pol II-like enzymes that evolved specialized roles in the production of noncoding transcripts for RNA silencing and genome defense.
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影响因子：
30.8
作者：
Dunoyer, Patrice;Himber, Christophe;Voinnet, Olivier
通讯作者：
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影响因子：
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DOI：
10.1073/pnas.0706701104
发表时间：
2007-09-11
影响因子：
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作者：
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