Leptin does not Induce an Inflammatory Response in the Murine Placenta
Leptin does not Induce an Inflammatory Response in the Murine Placenta
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瘦素不会引起小鼠胎盘的炎症反应
DOI：
10.1055/s-0034-1368710
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发表时间：
2014
影响因子：
2.2
通讯作者：
J. Dötsch
中科院分区：
文献类型：
--
作者：
E.-M. Turnwald;M.A. Alejandre-Alcazar;J. Ankerne;E. Rother;R. Kuschewski;M. Wohlfarth;C. Vohlen;M. Schnare;U. Meißner;J. Dötsch
瘦素被描述为脂肪组织中的促炎信号，它从脂肪细胞中释放出来，反过来激活免疫细胞。此外，已知瘦素水平在妊娠期间增加，并伴有胎盘炎症过程的增强。因此，我们假设瘦素的增加可能有助于诱导胎盘的炎症反应。为了验证这一假设，怀孕小鼠连续注射重组小鼠瘦素s.c。从第13天到第16天，导致母体循环血清瘦素水平增加3倍。采用qPCR方法检测胎盘中促炎细胞因子IL-6、tnf - α和抗炎细胞因子IL-10的表达。除了在瘦素刺激下略有升高的tnf - α外，没有发现其他变化。然而，瘦素处理小鼠胎盘中tnf - α蛋白水平并未显著升高。胎盘迷宫区白细胞浸润未见明显增加。总之，我们的数据首次证明，仅瘦素水平升高不会引起胎盘的炎症反应。
Leptin is described as a pro-inflammatory signal in fat tissue, which is released from adipocytes and in turn activates immune cells. Also, leptin levels are known to be increased in pregnancies complicated with enhanced inflammatory processes in the placenta. Hence, we assumed that increased leptin amounts might contribute to inducing an inflammatory response in the placenta. To test this hypothesis, pregnant mice were continuously infused with recombinant murine leptin s. c. from day g13 to g16, resulting in a 3-fold increase of maternal circulating serum leptin levels. Dissected placentas were examined for the expression of pro-inflammatory cytokines IL-6 and TNF-alpha and the anti-inflammatory cytokine IL-10 using qPCR analysis. No changes were found except for TNF-alpha, which was slightly elevated upon leptin stimulation. However, TNF-alpha protein levels were not significantly higher in placentas from leptin treated mice. Also, leukocyte infiltration in the labyrinth section of placentas was not increased. In summary, our data demonstrate for the first time that elevated leptin levels alone do not induce an inflammatory response in the placenta.
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胎盘中的瘦素
DOI：
--
发表时间：
2003
期刊：
影响因子：
--
作者：
D. Islami;P. Bischof
通讯作者：
P. Bischof
白色和棕色脂肪组织中瘦素和解偶联蛋白的免疫组织化学定位。
影响因子：
4.8
作者：
Cinti,S;Frederich,RC;Zingaretti,MC;DeMatteis,R;Flier,JS;Lowell,BB
通讯作者：
Lowell,BB
影响因子：
4.8
作者：
Rother, Eva;Kuschewski, Ruth;Doetsch, Joerg
通讯作者：
Doetsch, Joerg
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DOI：
--
发表时间：
2007
期刊：
J Clin Endocrinol Metab 92
影响因子：
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作者：
H.;Masuyama;H.;Nakatsukasa;N.;Takamoto;Y.;Hiramatrsu
通讯作者：
Hiramatrsu
影响因子：
--
作者：
Klaffenbach, Daniela;Meissner, Udo;Doetsch, Joerg
通讯作者：
Doetsch, Joerg