TGF-ß regulates enamel mineralization and maturation through KLK4 expression.
TGF-ß regulates enamel mineralization and maturation through KLK4 expression.
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DOI：
10.1371/journal.pone.0082267
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Kulkarni AB
中科院分区：
文献类型：
--
作者：
Cho A;Haruyama N;Hall B;Danton MJ;Zhang L;Arany P;Mooney DJ;Harichane Y;Goldberg M;Gibson CW;Kulkarni AB
Transforming growth factor-ß (TGF-ß) signaling plays an important role in regulating crucial biological processes such as cell proliferation, differentiation, apoptosis, and extracellular matrix remodeling. Many of these processes are also an integral part of amelogenesis. In order to delineate a precise role of TGF-ß signaling during amelogenesis, we developed a transgenic mouse line that harbors bovine amelogenin promoter-driven Cre recombinase, and bred this line with TGF-ß receptor II floxed mice to generate ameloblast-specific TGF-ß receptor II conditional knockout (cKO) mice. Histological analysis of the teeth at postnatal day 7 (P7) showed altered enamel matrix composition in the cKO mice as compared to the floxed mice that had enamel similar to the wild-type mice. The µCT and SEM analyses revealed decreased mineral content in the cKO enamel concomitant with increased attrition and thinner enamel crystallites. Although the mRNA levels remained unaltered, immunostaining revealed increased amelogenin, ameloblastin, and enamelin localization in the cKO enamel at the maturation stage. Interestingly, KLK4 mRNA levels were significantly reduced in the cKO teeth along with a slight increase in MMP-20 levels, suggesting that normal enamel maturation is regulated by TGF-ß signaling through the expression of KLK4. Thus, our study indicates that TGF-ß signaling plays an important role in ameloblast functions and enamel maturation.
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影响因子：
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