Basic fibroblast growth factor controls the expression and molecular structure of heparan sulfate in corneal endothelial cells.
Basic fibroblast growth factor controls the expression and molecular structure of heparan sulfate in corneal endothelial cells.
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碱性成纤维细胞生长因子控制角膜内皮细胞中硫酸乙酰肝素的表达和分子结构。
DOI：
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Eckhart Buddecke
中科院分区：
文献类型：
--
作者：
Annette Schmidt;Adriane Skaletz;Eckhart Buddecke
培养的牛角膜内皮细胞表达5-8 ng碱性成纤维细胞生长因子（bFGF）/mg细胞蛋白，并将其分布在细胞内和细胞周围区室之间。汇合培养物保留约80%的总bFGF细胞内，而20%是存在于细胞周围（胰蛋白酶释放）的隔间。在培养液中未检测到bFGF。1- 2ng/ml培养基中内源性或外源性（人重组）bFGF的存在足以支持细胞生长。同时，细胞将[35 S]硫酸盐和[3 H]葡糖胺掺入到与细胞层相关的硫酸化蛋白聚糖中，其速率是不存在bFGF时的三倍。蛋白聚糖合成的增强伴随着蛋白聚糖分布的变化。在对照细胞中，细胞相关的硫酸乙酰肝素占总糖胺聚糖的约30%，而在bFGF的影响下，硫酸乙酰肝素的量增加到约60%。同时，硫酸乙酰肝素分子的分子结构发生bFGF特异性变化，如肝素酶I降解产生的[35 S]寡糖模式所示。在bFGF的影响下，由于二糖和四糖，具有大于6个单糖的[35 S]寡糖的比例降低。用抗bFGF的中和抗体预处理bFGF可消除其生物活性。结果表明，角膜内皮细胞中膜相关硫酸乙酰肝素的合成速率和结构特征存在bFGF依赖性变化。硫酸乙酰肝素结构的修饰可以影响其bFGF结合和抗增殖活性。
Cultured bovine corneal endothelial cells express 5-8 ng basic fibroblast growth factor (bFGF)/mg cell protein and distribute it between the intracellular and pericellular compartment. Confluent cultures retain approximately 80% of the total bFGF intracellularly, whereas 20% is present in the pericellular (trypsin-releasable) compartment. No bFGF can be detected in the culture medium. The presence of 1-2 ng/ml medium of endogenous or exogenous (human recombinant) bFGF is sufficient to support cell growth. Simultaneously, cells incorporate [35S]sulfate and [3H]glucosamine into the sulfated proteoglycans associated with the cell layer at a rate that is three times higher than in the absence of bFGF. The enhanced proteoglycan synthesis is accompanied by a shift in proteoglycan distribution. In control cells, cell-associated heparan sulfate accounts for about 30% of the total glycosaminoglycans, whereas under the influence of bFGF the amount of heparan sulfate increases to approximately 60%. At the same time, the molecular structure of the heparan sulfate molecule undergoes bFGF-specific changes as indicated by the [35S]oligosaccharide pattern generated by heparitinase I degradation. The proportion of [35S]oligosaccharides with greater than six monosaccharides decreases on account of disaccharides and tetrasaccharides under the influence of bFGF. Pretreatment of bFGF with neutralizing antibodies against bFGF abolishes its biological activity. The results suggest a bFGF-dependent change in the rate of synthesis and structural features of the membrane-associated heparan sulfate in corneal endothelial cells. The modification of the heparan sulfate structure could influence its bFGF-binding and antiproliferative activity.
影响因子：
4
作者：
Jane Reiland;A. Rapraeger
通讯作者：
Jane Reiland;A. Rapraeger
DOI：
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发表时间：
1992
期刊：
The American journal of pathology
影响因子：
--
作者：
Yu,QC;McNeil,PL
通讯作者：
McNeil,PL
影响因子：
13.8
作者：
VLODAVSKY, I;BARSHAVIT, R;FUKS, Z
通讯作者：
FUKS, Z