An alkane monooxygenase (AlkB) family in which all electron transfer partners are covalently bound to the oxygen-activating hydroxylase.
An alkane monooxygenase (AlkB) family in which all electron transfer partners are covalently bound to the oxygen-activating hydroxylase.
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烷烃单加氧酶（ALKB）家族，其中所有电子转移伙伴都共价结合到氧气激活羟化酶。
DOI：
10.1016/j.jinorgbio.2021.111707
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发表时间：
2022-03
影响因子：
3.9
通讯作者：
Austin RN
中科院分区：
文献类型：
--
作者：
Williams SC;Luongo D;Orman M;Vizcarra CL;Austin RN
烷烃单加氧酶（AlkB）是一种催化烷烃羟基化的非血红素二铁酶。它通常存在于以烷烃为唯一碳和能量来源的链烷营养生物中。AlkB的活化通过其二铁活性位点的双电子还原发生，这促进了分子氧的结合、活化和裂解以插入惰性C-H键。电子通常由NADH通过红氧还蛋白还原酶（AlkT）提供给红氧还蛋白（AlkG）再提供给AlkB，尽管已经观察到替代的电子转移配偶体。在这里，我们报告了一个家庭的AlkBs中的两个电子传递伙伴（铁氧还蛋白和铁氧还蛋白还原酶）出现作为N-末端基因融合的羟化酶（ferr_ferrR_AlkB）。该酶催化中链烷烃（C6-C14）的羟基化，优选C10-C12。它只需要NADH的活动。它存在于许多已知为人类病原体的细菌中。对发现它的基因组邻域的调查表明，它可能参与烷烃代谢，可能促进病原体在非宿主环境中的生长。报道了铁氧还蛋白还原酶与铁氧还蛋白融合的烷烃单加氧酶的活性及其在生物学中的分布。
Alkane monooxygenase (AlkB) is a non-heme diiron enzyme that catalyzes the hydroxylation of alkanes. It is commonly found in alkanotrophic organisms that can live on alkanes as their sole source of carbon and energy. Activation of AlkB occurs via two-electron reduction of its diferric active site, which facilitates the binding, activation, and cleavage of molecular oxygen for insertion into an inert C-H bond. Electrons are typically supplied by NADH via a rubredoxin reductase (AlkT) to a rubredoxin (AlkG) to AlkB, although alternative electron transfer partners have been observed. Here we report a family of AlkBs in which both electron transfer partners (a ferredoxin and a ferredoxin reductase) appear as an N-terminal gene fusion to the hydroxylase (ferr_ferrR_AlkB). This enzyme catalyzes the hydroxylation of medium chain alkanes (C6-C14), with a preference for C10-C12. It requires only NADH for activity. It is present in a number of bacteria that are known to be human pathogens. A survey of the genome neighborhoods in which is it found suggest it may be involved in alkane metabolism, perhaps facilitating growth of pathogens in non-host environments. The activity and distribution in biology of alkane monooxygenases that have a fused ferredoxin reductase and ferredoxin are reported.
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影响因子：
5.2
作者：
Bertrand EM;Keddis R;Groves JT;Vetriani C;Austin RN
通讯作者：
Austin RN
影响因子：
3.9
作者：
Bertrand, E;Sakai, R;Austin, RN
通讯作者：
Austin, RN
影响因子：
3.2
作者：
Sabirova, Julia S.;Ferrer, Manuel;Golyshin, Peter N.
通讯作者：
Golyshin, Peter N.
微生物和环境中的多种烷烃羟化酶基因
影响因子：
4.6
作者：
Nie Y;Chi CQ;Fang H;Liang JL;Lu SL;Lai GL;Tang YQ;Wu XL
通讯作者：
Wu XL
影响因子：
4.8
作者：
Mitchell, AG;Martin, CE
通讯作者：
Martin, CE