The α(2)δ Calcium Channel Subunit Accessorily and Independently Affects the Biological Function of Ditylenchus destructor.
The α(2)δ Calcium Channel Subunit Accessorily and Independently Affects the Biological Function of Ditylenchus destructor.
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α2α钙通道亚基辅助且独立地影响腐烂茎线虫的生物学功能
DOI：
10.3390/ijms232112999
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发表时间：
2022-10-27
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
α2δ亚基是一种高电压激活（HVA）钙通道（Cav 1和Cav 2）辅助亚基，可增加哺乳动物质膜中HVA钙通道的密度和功能。然而，其在植物寄生线虫中的功能仍然未知。本研究克隆了腐烂茎线虫电压门控钙通道（VGCC）α2δ亚基的全长cDNA序列（命名为DdCavα2δ）。结果表明，DdCavα2δ基因在卵期表达，在J3期表达最少。RNA-DIG原位杂交结果表明，DdCavα2δ亚基在杜氏田鼠体壁、食道腺、子宫、子宫后和骨针中均有表达。析构函数RNA干扰（RNAi）在体外的应用影响了果蝇的运动、生殖、趋化性、口针刺入和蛋白质分泌。DdCα1D、DdCα1A和DdCavα2δ在J3期分别对DdCα1D、DdCα1A和DdCavα2δ有不同程度的破坏作用。基于RNAi实验的结果，我们推测L型VGCC可能影响D.析构函数非L型VGCC可能影响D.析构函数DdCavα2δ亚基基因还影响了D.析构函数这些结果揭示了VGCC α2δ亚基在D.同时也为植物寄生线虫VGCC的进一步研究提供了理论基础。
The α2δ subunit is a high-voltage activated (HVA) calcium channel (Cav1 and Cav2) auxiliary subunit that increases the density and function of HVA calcium channels in the plasma membrane of mammals. However, its function in plant parasitic nematodes remains unknown. In this study, we cloned the full-length cDNA sequence of the voltage-gated calcium channel (VGCC) α2δ subunit (named DdCavα2δ) in Ditylenchus destructor. We found that DdCavα2δ tends to be expressed in the egg stage, followed by the J3 stage. RNA-DIG in situ hybridization experiments showed that the DdCavα2δ subunit was expressed in the body wall, esophageal gland, uterus, post uterine, and spicules of D. destructor. The in vitro application of RNA interference (RNAi) affected the motility, reproduction, chemotaxis, stylet thrusting, and protein secretion of D. destructor to different degrees by targeting DdCα1D, DdCα1A, and DdCavα2δ in J3 stages, respectively. Based on the results of RNAi experiments, it was hypothesized that L-type VGCC may affect the motility, chemotaxis, and stylet thrusting of D. destructor. Non-L-type VGCC may affect the protein secretion and reproduction of D. destructor. The DdCavα2δ subunit gene also affected the motility, chemotaxis, and reproduction of D. destructor. These findings reveal the independent function of the VGCC α2δ subunit in D. destructor as well as give a theoretical foundation for future research on plant parasitic nematode VGCC.
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影响因子：
4.8
作者：
Kimber, Michael J.;McKinney, Susan;Maule, Aaron G.
通讯作者：
Maule, Aaron G.
影响因子：
5.3
作者：
Barclay, J;Balaguero, N;Rees, M
通讯作者：
Rees, M
DOI：
10.1523/jneurosci.2519-09.2009
发表时间：
2009-09-02
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Guzman JN;Sánchez-Padilla J;Chan CS;Surmeier DJ
通讯作者：
Surmeier DJ
影响因子：
7.3
作者：
Dissanayake, VUK;Gee, NS;Woodruff, GN
通讯作者：
Woodruff, GN
影响因子：
0.8
作者：
Kud, Joanna;Solo, Nejra;Xiao, Fangming
通讯作者：
Xiao, Fangming