Radioprotection of hematopoietic tissues in mice by indomethacin.
Radioprotection of hematopoietic tissues in mice by indomethacin.
复制标题
吲哚美辛对小鼠造血组织的辐射保护作用。
作者：
I. Nishiguchi;Y. Furuta;N. Hunter;D. Murray;L. Milas
我们最近报道，前列腺素 (PG) 合成抑制剂吲哚美辛可增加产生 PG 的小鼠肿瘤的放射反应，但它可以保护造血系统免受辐射损伤 [Furuta et al., Cancer Res. 48、3008-3013（1988）]。在这里，我们研究了吲哚美辛辐射防护作用的可能机制。在外源性脾集落测定中，接受吲哚美辛治疗的小鼠的骨髓细胞与未接受吲哚美辛治疗的小鼠的骨髓细胞表现出相似的放射反应，因此排除了真正的放射防护机制。此外，骨髓细胞总数和骨髓干细胞数量均未受到吲哚美辛治疗的影响。然而，吲哚美辛会引起明显的脾肿大，这与脾脏中有核细胞和造血干细胞数量的增加有关。后者通过外源性脾集落测定来确定。因此，吲哚美辛通过刺激脾脏中的造血细胞间接保护造血组织。根据内源性脾脏集落测定，当吲哚美辛与真正的放射防护剂 WR-2721 联合使用时，我们获得了比单独使用任何一种都更好的放射防护效果。
We recently reported that indomethacin, an inhibitor of prostaglandin (PG) synthesis, increased the radioresponse of PG-producing murine tumors, but it protected the hematopoietic system from radiation damage [Furuta et al., Cancer Res. 48, 3008-3013 (1988)]. Here we have investigated possible mechanisms responsible for the radioprotective effect of indomethacin. In the exogenous spleen colony assay, bone marrow cells from indomethacin-treated mice showed a similar radioresponse to those from mice not treated with indomethacin, thus excluding true radioprotection as a mechanism. Also, neither the total number of bone marrow cells nor the number of stem cells in bone marrow were affected by the treatment with indomethacin. However, indomethacin induced significant splenomegaly, which was associated with an increased number of both nucleated cells and hematopoietic stem cells in the spleen. The latter was determined by the exogenous spleen colony assay. Thus indomethacin protected hematopoietic tissue indirectly through stimulation of hematopoietic cells in the spleen. When indomethacin was combined with WR-2721, which is a true radioprotector, we obtained a greater radioprotective effect than with either used alone according to the endogenous spleen colony assay.
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影响因子：
11.2
作者：
L. Milas;N. Hunter;B. Reid;H. Thames
通讯作者：
L. Milas;N. Hunter;B. Reid;H. Thames
用吲哚美辛治疗可增加小鼠肿瘤的放射反应。
影响因子：
11.2
作者：
Furuta,Y;Hunter,N;BarkleyJr,T;Hall,E;Milas,L
通讯作者：
Milas,L
S-2-(3-氨基丙氨基)乙基硫代磷酸对小鼠辐射引起的腿部挛缩的保护作用。
影响因子：
11.2
作者：
Hunter,N;Milas,L
通讯作者：
Milas,L
肿瘤大小对 S-2-(3-氨基丙氨基)乙基硫代磷酸和米索硝唑改变肿瘤对环磷酰胺反应的影响。
影响因子：
11.2
作者：
Milas,L;Ito,H;Hunter,N
通讯作者：
Hunter,N
S-2-(3-氨基丙氨基)乙基硫代磷酸可防止辐射和环磷酰胺诱导的抗肿瘤耐药性减弱。
影响因子：
11.2
作者：
Milas,L;McBride,WH;Hunter,N;Ito,H
通讯作者：
Ito,H