Lateral line ablation by ototoxic compounds results in distinct rheotaxis profiles in larval zebrafish.
Lateral line ablation by ototoxic compounds results in distinct rheotaxis profiles in larval zebrafish.
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DOI：
10.1038/s42003-023-04449-2
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发表时间：
2023-01-21
影响因子：
5.9
通讯作者：
中科院分区：
文献类型：
--
作者：
斑马鱼侧线是毛细胞器官损伤的既定模型，但很少有研究将机械破坏与生物学相关行为的变化联系起来。我们使用斑马鱼幼虫来确定耳毒性化合物的损害如何影响趋流性。幼虫用硫酸铜或新霉素处理以破坏侧线功能，然后暴露于水流刺激。通过视频记录它们的游泳行为，然后分别使用DeepLabCut和SimBA软件来跟踪运动和分类趋流性行为。侧线中断鱼进行趋流性，但他们游更大的距离，持续时间更短，更大的角度变化比对照组。此外，光谱分解分析证实，病变的鱼表现出耳毒性化合物的特定行为配置文件的幅度，频率和线性和角运动的波动之间的相互关系的明显变化。我们的观察结果表明，侧线输入是需要鱼有效地保持其站在流，并揭示了常用的病变方法具有独特的影响趋流行为。幼斑马鱼进行耳毒性化合物，以评估其对趋流性的影响，和化合物的特定行为配置文件揭示了独特的影响斑马鱼趋流性行为。
The zebrafish lateral line is an established model for hair cell organ damage, yet few studies link mechanistic disruptions to changes in biologically relevant behavior. We used larval zebrafish to determine how damage via ototoxic compounds impact rheotaxis. Larvae were treated with CuSO4 or neomycin to disrupt lateral line function then exposed to water flow stimuli. Their swimming behavior was recorded on video then DeepLabCut and SimBA software were used to track movements and classify rheotaxis behavior, respectively. Lateral line-disrupted fish performed rheotaxis, but they swam greater distances, for shorter durations, and with greater angular variance than controls. Furthermore, spectral decomposition analyses confirmed that lesioned fish exhibited ototoxic compound-specific behavioral profiles with distinct changes in the magnitude, frequency, and cross-correlation between fluctuations in linear and angular movements. Our observations demonstrate that lateral line input is needed for fish to hold their station in flow efficiently and reveals that commonly used lesion methods have unique effects on rheotaxis behavior. Larval zebrafish are subjected to ototoxic compounds to evaluate their impact on rheotaxis, and compound-specific behaviour profiles reveal unique effects on zebrafish rheotaxis behavior.
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DOI：
10.1007/s10162-002-3022-x
发表时间：
2003-06-01
影响因子：
2.4
作者：
Harris, JA;Cheng, AG;Rubel, EW
通讯作者：
Rubel, EW
DOI：
10.1007/2506_2013_15
发表时间：
2014-01-01
期刊：
LATERAL LINE SYSTEM
影响因子：
--
作者：
Chagnaud, Boris Philippe;Coombs, Sheryl
通讯作者：
Coombs, Sheryl
影响因子：
2.8
作者：
Buck, Lauren M. J.;Winter, Matthew J.;Whitfield, Tanya T.
通讯作者：
Whitfield, Tanya T.
影响因子：
2.8
作者：
Fitak, Robert R.;Johnsen, Sonke
通讯作者：
Johnsen, Sonke
影响因子：
3.5
作者：
Johansen, J. L.;Fulton, C. J.;Bellwood, D. R.
通讯作者：
Bellwood, D. R.