Affinity- and activity-based probes synthesized from structurally diverse hops-derived xanthohumol flavonoids reveal highly varied protein profiling in Escherichia coli.
Affinity- and activity-based probes synthesized from structurally diverse hops-derived xanthohumol flavonoids reveal highly varied protein profiling in Escherichia coli.
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作者：
Xanthohumol, the principle prenylflavonoid found in hops (Humulus lupulus) and a reported anti-inflammatory agent, has great potential for pharmaceutical interventions related to inflammatory disorders in the gut. A suite of probes was prepared from xanthohumol and its structural isomer isoxanthohumol to enable profiling of both protein affinity binding and catalytic enzyme reactivity. The regiochemistry of the reactive group on the probes was altered to reveal how probe structure dictates protein labeling, and which probes best emulate the natural flavonoids. Affinity- and activity-based probes were applied to Escherichia coli, and protein labeling was measured by chemoproteomics. Structurally dependent activity-based probe protein labeling demonstrates how subtle alterations in flavonoid structure and probe reactive groups can result in considerably different protein interactions. This work lays the groundwork to expand upon unexplored cellular activities related to xanthohumol interactions, metabolism, and anti-inflammatory mechanisms. Xanthohumol, the principle prenylflavonoid found in hops (Humulus lupulus) and a reported anti-inflammatory agent, has great potential for pharmaceutical interventions related to inflammatory disorders in the gut.
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影响因子：
5.6
作者：
Paraiso IL;Tran TQ;Magana AA;Kundu P;Choi J;Maier CS;Bobe G;Raber J;Kioussi C;Stevens JF
通讯作者：
Stevens JF
DOI：
10.3390/antibiotics10040392
发表时间：
2021-04-06
期刊：
Antibiotics (Basel, Switzerland)
影响因子：
--
作者：
Sleha R;Radochova V;Mikyska A;Houska M;Bolehovska R;Janovska S;Pejchal J;Muckova L;Cermak P;Bostik P
通讯作者：
Bostik P
影响因子：
4.3
作者：
Benkherouf AY;Eerola K;Soini SL;Uusi-Oukari M
通讯作者：
Uusi-Oukari M
影响因子：
3.5
作者：
Dubinsky, Luba;Krom, Bastiaan P.;Meijler, Michael M.
通讯作者：
Meijler, Michael M.
影响因子：
16.6
作者：
Gall, Mechthild;Thomsen, Maren;Bornscheuer, Uwe T.
通讯作者：
Bornscheuer, Uwe T.