The effect of the fibre orientation of electrospun scaffolds on the matrix production of rabbit annulus fibrosus-derived stem cells.
The effect of the fibre orientation of electrospun scaffolds on the matrix production of rabbit annulus fibrosus-derived stem cells.
复制标题
静电纺支架的纤维取向对兔纤维环干细胞基质生成的影响
DOI：
10.1038/boneres.2015.12
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发表时间：
2015
期刊：
影响因子：
12.7
通讯作者：
Li, Bin
中科院分区：
文献类型：
--
作者：
Liu, Chen;Zhu, Caihong;Li, Jun;Zhou, Pinghui;Chen, Min;Yang, Huilin;Li, Bin
纤维环（AF）组织工程作为椎间盘（IVD）退变的治疗最近受到越来越多的关注;然而，由于AF组织的显著复杂性，这种工程仍然具有挑战性。为了设计功能性AF替代物，模拟天然AF组织的细胞、生物化学和结构特征的细胞支架构建体的制造是至关重要的。在这项研究中，我们使用静电纺丝技术制造了对齐的纤维聚氨酯支架，并将其用于培养AF衍生的干/祖细胞（AFSC）。随机纤维支架，也通过静电纺丝制备，被用作对照。我们比较了排列支架和随机支架上AFSCs的形态、增殖、基因表达和基质生成。细胞在排列支架和随机支架上的贴壁和增殖没有明显差异。然而，与随机支架上的细胞相比，对齐支架上的AFSC更长，排列更好，并且它们表现出更高的基因表达和胶原蛋白-I和聚集蛋白聚糖的基质产生。两组之间胶原蛋白II的基因表达和蛋白质产生似乎没有差异。总之，这些发现表明，对齐的纤维支架可以提供一个有利的微环境，为AFSC分化成细胞类似于外部AF细胞，主要产生胶原蛋白-I基质。
Annulus fibrosus (AF) tissue engineering has recently received increasing attention as a treatment for intervertebral disc (IVD) degeneration; however, such engineering remains challenging because of the remarkable complexity of AF tissue. In order to engineer a functional AF replacement, the fabrication of cell-scaffold constructs that mimic the cellular, biochemical and structural features of native AF tissue is critical. In this study, we fabricated aligned fibrous polyurethane scaffolds using an electrospinning technique and used them for culturing AF-derived stem/progenitor cells (AFSCs). Random fibrous scaffolds, also prepared via electrospinning, were used as a control. We compared the morphology, proliferation, gene expression and matrix production of AFSCs on aligned scaffolds and random scaffolds. There was no apparent difference in the attachment or proliferation of cells cultured on aligned scaffolds and random scaffolds. However, compared to cells on random scaffolds, the AFSCs on aligned scaffolds were more elongated and better aligned, and they exhibited higher gene expression and matrix production of collagen-I and aggrecan. The gene expression and protein production of collagen-II did not appear to differ between the two groups. Together, these findings indicate that aligned fibrous scaffolds may provide a favourable microenvironment for the differentiation of AFSCs into cells similar to outer AF cells, which predominantly produce collagen-I matrix.
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影响因子：
2.4
作者：
Li, Bin;Lin, Michael;Tang, Ying;Wang, Bing;Wang, James H. -C.
通讯作者：
Wang, James H. -C.
影响因子：
5.3
作者：
Guo Q;Liu C;Li J;Zhu C;Yang H;Li B
通讯作者：
Li B
影响因子：
3.7
作者：
Li J;Liu C;Guo Q;Yang H;Li B
通讯作者：
Li B
DOI：
10.3390/s101109948
发表时间：
2010
期刊：
Sensors (Basel, Switzerland)
影响因子：
--
作者：
Li B;Wang JH
通讯作者：
Wang JH
影响因子：
3
作者：
MARCHAND, F;AHMED, AM
通讯作者：
AHMED, AM