GmFT2a and GmFT5a redundantly and differentially regulate flowering through interaction with and upregulation of the bZIP transcription factor GmFDL19 in soybean.
GmFT2a and GmFT5a redundantly and differentially regulate flowering through interaction with and upregulation of the bZIP transcription factor GmFDL19 in soybean.
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GmFT2a 和 GmFT5a 通过与大豆中 bZIP 转录因子 GmFDL19 的相互作用和上调来冗余且差异性地调节开花
DOI：
10.1371/journal.pone.0097669
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Kong F
中科院分区：
文献类型：
--
作者：
Nan H;Cao D;Zhang D;Li Y;Lu S;Tang L;Yuan X;Liu B;Kong F
开花位点T（开花位点T）是拟南芥（Arabidopsis thaliana）中关键的开花整合子，其同源物在许多植物物种中编码开花因子，而不受其光周期响应的影响。两个FT同源基因GmFT2a和GmFT5a参与大豆的光周期调控开花，并协调控制开花。然而，对GmFT2a和GmFT5a在大豆光周期调控开花中的作用的分子和遗传学认识非常有限。在本研究中，我们证明GmFT2a和GmFT5a能够在非诱导长日照（LD）条件下在大豆品种威廉姆斯82中过表达这两个基因，从而促进大豆早花。GmFT2a和GmFT5a对GmAP1、GmSOC1和GmLFY等几个花鉴定基因的大豆同源基因以冗余和差异的方式显著上调。bZIP转录因子GmFDL19与GmFT2a和GmFT5a相互作用，并通过酵母双杂交和双分子荧光互补（BiFC）证实了这种相互作用。GmFDL19在大豆中的过表达导致开花提前，并且GmFDL19也上调了开花鉴定基因的转录水平，这与GmFT2a和GmFT5a的上调一致。GmFT2a也能诱导GmFDL19的转录。电泳迁移率转移试验（EMSA）结果表明，GmFDL19能够与GmAP1a启动子中的顺式元件结合。综上所述，我们的研究结果表明，GmFT2a和GmFT5a通过与bZIP转录因子GmFDL19的物理相互作用和转录上调，冗余和差异控制大豆光周期调控的开花，从而诱导花特性基因的表达。
FLOWERING LOCUS T (FT) is the key flowering integrator in Arabidopsis (Arabidopsis thaliana), and its homologs encode florigens in many plant species regardless of their photoperiodic response. Two FT homologs, GmFT2a and GmFT5a, are involved in photoperiod-regulated flowering and coordinately control flowering in soybean. However, the molecular and genetic understanding of the roles played by GmFT2a and GmFT5a in photoperiod-regulated flowering in soybean is very limited. In this study, we demonstrated that GmFT2a and GmFT5a were able to promote early flowering in soybean by overexpressing these two genes in the soybean cultivar Williams 82 under noninductive long-day (LD) conditions. The soybean homologs of several floral identity genes, such as GmAP1, GmSOC1 and GmLFY, were significantly upregulated by GmFT2a and GmFT5a in a redundant and differential pattern. A bZIP transcription factor, GmFDL19, was identified as interacting with both GmFT2a and GmFT5a, and this interaction was confirmed by yeast two-hybridization and bimolecular fluorescence complementation (BiFC). The overexpression of GmFDL19 in soybean caused early flowering, and the transcription levels of the flowering identity genes were also upregulated by GmFDL19, as was consistent with the upregulation of GmFT2a and GmFT5a. The transcription of GmFDL19 was also induced by GmFT2a. The results of the electrophoretic mobility shift assay (EMSA) indicated that GmFDL19 was able to bind with the cis-elements in the promoter of GmAP1a. Taken together, our results suggest that GmFT2a and GmFT5a redundantly and differentially control photoperiod-regulated flowering in soybean through both physical interaction with and transcriptional upregulation of the bZIP transcription factor GmFDL19, thereby inducing the expression of floral identity genes.
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影响因子：
2.3
作者：
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通讯作者：
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