Spontaneous production of PDGF A-chain homodimer by rat lung fibroblasts in vitro.
Spontaneous production of PDGF A-chain homodimer by rat lung fibroblasts in vitro.
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体外大鼠肺成纤维细胞自发产生 PDGF A 链同二聚体。
DOI：
10.1152/ajplung.1992.263.2.l185
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Kelley,J
中科院分区：
文献类型：
--
作者：
Fabisiak,JP;Absher,M;Evans,JN;Kelley,J
血小板衍生生长因子（PDGF）被认为是肺内成纤维细胞生长和表型的决定性介质。肺内PDGF的细胞来源仍不明确。因此，研究了肺成纤维细胞自身在体外产生PDGF的能力。北方和Western印迹分析显示，PDGF-A mRNA的表达和分泌的A链蛋白的成纤维细胞来源于成年和胎鼠肺。PDGF-A基因或蛋白表达低于以类似方式检查的两种人肺成纤维细胞系的检测限。在检查的任何肺成纤维细胞系中均未检测到PDGF-B转录物或蛋白质。从这些相同的肺成纤维细胞系收集条件培养基（CM），并使用人胎儿肺成纤维细胞作为靶来测试其促进细胞生长的能力。成年和胎鼠肺成纤维细胞被发现产生一个强大的和有效的刺激细胞生长。大鼠成纤维细胞来源的CM中的促生长活性作为“感受态”因子起作用，并被抗PDGF抗体部分抑制。因此，大鼠肺成纤维细胞在体外产生有效的生长因子，其中至少有一个似乎是PDGF-AA。PDGF-AA在大鼠和人肺成纤维细胞中的表达存在差异。在体内，产生生长因子的成纤维细胞可能通过产生PDGF-AA在肺修复和重塑中发挥作用。
Platelet-derived growth factor (PDGF) is considered a decisive mediator of fibroblast growth and phenotype within the lung. The cellular sources of PDGF within the lung remain undefined. The ability of lung fibroblasts themselves to produce PDGF in vitro was therefore investigated. Northern and Western blot analyses revealed the expression of PDGF-A mRNA and secretion of A-chain containing proteins by fibroblasts derived from adult and fetal rat lung. PDGF-A gene or protein expression were below the limits of detection in two human lung fibroblast lines examined in a similar manner. PDGF-B transcripts or proteins were not detected in any lung fibroblast line examined. Conditioned medium (CM) was collected from these same lung fibroblast lines and tested for its ability to promote cell growth using human fetal lung fibroblasts as targets. Both adult and fetal rat lung fibroblasts were found to produce a potent and efficacious stimulus for cell growth. Growth-promoting activity in rat fibroblast-derived CM functioned as a "competence" factor and was partially inhibited by anti-PDGF antibody. Thus rat lung fibroblasts in vitro produce potent growth factors of which at least one appears to be PDGF-AA. Differences in the expression of PDGF-AA between rat and human lung fibroblasts exist. Growth factor-producing fibroblasts may play a role in lung repair and remodeling through production PDGF-AA in vivo.
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理解中的认知过程
DOI：
--
发表时间：
1977
期刊：
影响因子：
--
作者：
M. Just;P. Carpenter
通讯作者：
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DOI：
10.2307/1421786
发表时间：
1974
期刊：
--
影响因子：
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DOI：
--
发表时间：
1976
期刊：
影响因子：
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DOI：
10.1177/016146812502700305
发表时间：
1925
期刊：
Teachers College Record: The Voice of Scholarship in Education
影响因子：
--
作者：
W. Mccall;Lelah Mae Crabbs
通讯作者：
Lelah Mae Crabbs
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影响因子：
24.8
作者：
Johnson,MK;Hasher,L
通讯作者：
Hasher,L