Genetic methods to identify and manipulate newly born neurons in the adult brain.
Genetic methods to identify and manipulate newly born neurons in the adult brain.
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DOI：
10.3389/fnins.2011.00064
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发表时间：
2011
影响因子：
4.3
通讯作者：
Kageyama R
中科院分区：
文献类型：
--
作者：
Imayoshi I;Sakamoto M;Kageyama R
尽管哺乳动物的神经发生大部分在围产期完成，但在侧脑室的室下区和海马齿状回的颗粒下区不断产生新的神经元。自从发现成体神经发生以来，人们对成体神经发生的各个方面进行了许多广泛的研究，包括成体神经干细胞的增殖和命运特异性，以及新生神经元的迁移、成熟和突触整合。此外，最近的研究揭示了成人神经发生对嗅觉相关和海马介导的大脑功能的强烈贡献。由于技术进步促进了成人中枢神经系统数十亿预先存在的神经元中新生神经元的识别和选择性操作，成人神经发生领域取得了巨大进展。在这篇综述中，我们介绍了可视化新生成的神经元和操纵成人大脑神经发生的方法学的最新进展。特别是，进一步详细讨论了位点特异性重组酶和 Tet 诱导系统与转基因或基因靶向策略相结合的应用。
Although mammalian neurogenesis is mostly completed by the perinatal period, new neurons are continuously generated in the subventricular zone of the lateral ventricle and the subgranular zone of the hippocampal dentate gyrus. Since the discovery of adult neurogenesis, many extensive studies have been performed on various aspects of adult neurogenesis, including proliferation and fate-specification of adult neural stem cells, and the migration, maturation and synaptic integration of newly born neurons. Furthermore, recent research has shed light on the intensive contribution of adult neurogenesis to olfactory-related and hippocampus-mediated brain functions. The field of adult neurogenesis progressed tremendously thanks to technical advances that facilitate the identification and selective manipulation of newly born neurons among billions of pre-existing neurons in the adult central nervous system. In this review, we introduce recent advances in the methodologies for visualizing newly generated neurons and manipulating neurogenesis in the adult brain. Particularly, the application of site-specific recombinases and Tet inducible system in combination with transgenic or gene targeting strategy is discussed in further detail.
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影响因子：
48
作者：
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