Multiomics global landscape of stemness-related gene clusters in adipose-derived mesenchymal stem cells
Multiomics global landscape of stemness-related gene clusters in adipose-derived mesenchymal stem cells
复制标题
脂肪间充质干细胞中干性相关基因簇的多组学全球景观
DOI：
10.1186/s13287-020-01823-3
复制
发表时间：
2020-07
影响因子：
7.5
通讯作者：
Li Hong-Mian
中科院分区：
文献类型：
--
作者：
Lu Guan-Ming;Rong Yong-Xian;Liang Zhi-Jie;Hunag Dong-Lin;Ma Yan-Fei;Luo Zhi-Zhai;Wu Fang-Xiao;Liu Xin-Heng;Liu Yu;Mo Steven;Qi Zhong-Quan;Li Hong-Mian
背景
脂肪来源的间充质干细胞（AD - MSCs）是一种丰富且广泛应用的干细胞类型。来自不同年龄供体的不同传代的AD - MSCs的分子特征尚未得到很好的阐明。
方法
六（此处“Six”可能是不完整的内容，如果还有后续信息，请提供完整以便更准确地翻译）
BackgroundAdipose-derived mesenchymal stem cells (AD-MSCs) are a type of stem cell that is abundant and widely used. The molecular characteristics of AD-MSCs from different passages from donors of different ages have not been well elucidated.MethodsSix kinds of AD-MSCs ((E1, E2, E3, Y1, Y2, and Y3) with E denoting cells derived from an elderly patient, Y denoting cells derived from a young patient, and 1, 2, and 3 representing passages 3, 6, and 10) were obtained from human abdominal adipose tissue. We obtained the protein expression profile, the mRNA expression profile, the lncRNA expression profile, and the methylation profile of each kind of AD-MSC by sequencing. After calculating the stemness indices, genes related to stemness were extracted. The multiomics correlation analysis was performed in the stemness-related genes. In addition, short time-series expression miner (STEM) analysis was performed for all cell passages and donor ages. To further explore the biological functions of the stemness-related genes, we performed Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. Finally, the lncRNA-KEGG network and transcription factor (TF)-KEGG network were constructed based on the RNAInter database and TRRUST v2 database.ResultsThe stemness of the Y1, E1, and Y2 cells was higher than that of the E2, Y3, and E3 cells. The stemness was the highest for Y1 cells and the lowest for E3 cells. STEM analysis showed that five stemness-related gene clusters were associated with the cell passages, and only one gene cluster was associated with age. The enrichment analysis results showed that the biological processes (BPs) and KEGG pathways were mainly involved in the proliferation, differentiation, and migration of cells. The global regulatory landscape of AD-MSCs was constructed: 25 TFs and 16 lncRNAs regulated 21 KEGG pathways through 27 mRNAs. Furthermore, we obtained a core stemness-related gene set consisting of ITGAV, MAD2L1, and PCNA. These genes were expressed at higher levels in Y1 cells than in E3 cells.ConclusionThe multiomics global landscape of stemness-related gene clusters was determined for AD-MSCs, which may be helpful for selecting AD-MSCs with increased stemness.
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影响因子：
5.3
作者：
通讯作者：
--
影响因子：
3.1
作者：
H. Tsuruga;N. Yabuta;Katsuhito Hashizume;Masako Ikeda;Yuichi Endo;Hiroshi Nojima
通讯作者：
H. Tsuruga;N. Yabuta;Katsuhito Hashizume;Masako Ikeda;Yuichi Endo;Hiroshi Nojima
影响因子：
14.9
作者：
Ritchie ME;Phipson B;Wu D;Hu Y;Law CW;Shi W;Smyth GK
通讯作者：
Smyth GK
DOI：
10.1016/bs.enz.2016.03.003
发表时间：
2016
期刊：
The Enzymes
影响因子：
--
作者：
Boehm EM;Gildenberg MS;Washington MT
通讯作者：
Washington MT
影响因子：
64.5
作者：
Malta TM;Sokolov A;Gentles AJ;Burzykowski T;Poisson L;Weinstein JN;Kamińska B;Huelsken J;Omberg L;Gevaert O;Colaprico A;Czerwińska P;Mazurek S;Mishra L;Heyn H;Krasnitz A;Godwin AK;Lazar AJ;Cancer Genome Atlas Research Network;Stuart JM;Hoadley KA;Laird PW;Noushmehr H;Wiznerowicz M
通讯作者：
Wiznerowicz M