Strain prioritization for natural product discovery by a high-throughput real-time PCR method.
Strain prioritization for natural product discovery by a high-throughput real-time PCR method.
复制标题
DOI：
10.1021/np5006168
复制
发表时间：
2014-10-24
影响因子：
5.1
通讯作者：
Shen, Ben
中科院分区：
文献类型：
--
作者：
Hindra;Huang, Tingting;Yang, Dong;Rudolf, Jeffrey D.;Xie, Pengfei;Xie, Guangbo;Teng, Qihui;Lohman, Jeremy R.;Zhu, Xiangcheng;Huang, Yong;Zhao, Li-Xing;Jiang, Yi;Duan, Yanwen;Shen, Ben
Natural products offer unmatched chemical and structural diversity compared to other small-molecule libraries, but traditional natural product discovery programs are not sustainable, demanding too much time, effort, and resources. Here we report a strain prioritization method for natural product discovery. Central to the method is the application of real-time PCR, targeting genes characteristic to the biosynthetic machinery of natural products with distinct scaffolds in a high-throughput format. The practicality and effectiveness of the method were showcased by prioritizing 1911 actinomycete strains for diterpenoid discovery. A total of 488 potential diterpenoid producers were identified, among which six were confirmed as platensimycin and platencin dual producers and one as a viguiepinol and oxaloterpin producer. While the method as described is most appropriate to prioritize strains for discovering specific natural products, variations of this method should be applicable to the discovery of other classes of natural products. Applications of genome sequencing and genome mining to the high-priority strains could essentially eliminate the chance elements from traditional discovery programs and fundamentally change how natural products are discovered.
登录
查看更多内容
影响因子：
16.6
作者：
Jayasuriya, Hiranthi;Herath, Kithsiri B.;Singh, Sheo B.
通讯作者：
Singh, Sheo B.
影响因子：
7.8
作者：
Smanski, Michael J.;Peterson, Ryan M.;Huang, Sheng-Xiong;Shen, Ben
通讯作者：
Shen, Ben
影响因子：
5.1
作者：
Xie P;Ma M;Rateb ME;Shaaban KA;Yu Z;Huang SX;Zhao LX;Zhu X;Yan Y;Peterson RM;Lohman JR;Yang D;Yin M;Rudolf JD;Jiang Y;Duan Y;Shen B
通讯作者：
Shen B
影响因子：
3.4
作者：
Bachmann, Brian O.;Van Lanen, Steven G.;Baltz, Richard H.
通讯作者：
Baltz, Richard H.
DOI：
10.1073/pnas.0337542100
发表时间：
2003-02-18
影响因子：
11.1
作者：
Gust, B;Challis, GL;Chater, KF
通讯作者：
Chater, KF