Predominance of non-Streptococcus mutans bacteria in dental biofilm and its relation to caries progression.
Predominance of non-Streptococcus mutans bacteria in dental biofilm and its relation to caries progression.
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DOI：
10.1016/j.sjbs.2021.08.052
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发表时间：
2021-12
影响因子：
4.4
通讯作者：
Alagl AS
中科院分区：
文献类型：
--
作者：
AlEraky DM;Madi M;El Tantawi M;AlHumaid J;Fita S;AbdulAzeez S;Borgio JF;Al-Harbi FA;Alagl AS
这项研究旨在评估低龋者和高龋者之间生物被膜细菌组成的差异。纳入的患者没有医疗问题，在过去3个月内没有抗生素使用、漱口或氟化物应用的病史。龋齿记录在空化水平；得分按全国平均值计算(DMFT为4.8，DMFT为2.7)。从近中、远中、颊、舌面和咬合面收集生物膜样本。根据龋病经验，将个体分为低龋组和高龋组，比较两组用16S rRNA基因测序鉴定的细菌，并对分离株进行分子系统发育分析。共随机抽取27个低(n=13)和高(n=414)个龋齿样本。用16S rRNA序列对口腔细菌进行鉴定，在低龋者中检出粘液玫瑰杆菌和黑斑杆菌，而在高龋者中检出牙本质假单胞菌。两株链球菌。在高龋者中仅检出血链球菌、米蒂斯链球菌、中华链球菌、红链球菌、前庭链球菌、克里斯滕链球菌和马西里氏链球菌。这项研究揭示了高龋组中粘液杆菌的缺失及其与低龋组的共存。变形链球菌对龋病的贡献率不高，而血链球菌是沙特高龋病患者的主要致龋菌。
This study aims to assess differences in biofilm bacterial composition between patients with low and high caries. Patients without a medical problem and with no history of antibiotic use, mouth wash or fluoride application in the previous 3 months were recruited. Caries was recorded at cavitation level; score was calculated by a national mean (dmft of 4.8 and DMFT of 2.7). Pooled biofilm samples were collected from mesial, distal, buccal, lingual, and occlusal surfaces. Based on caries experience, individuals were classified into low and high caries and both groups were compared regarding bacteria identified using 16S rRNA gene sequencing, and molecular phylogenetic analysis of the isolates was performed. A total of twenty seven randomly selected samples with low (n = 13) and high (n = 14) caries. Identification of oral bacteria was performed using 16S rRNA sequence, Rothia mucilaginosa and R. aeria were identified in low caries individuals, while R. dentocariosa was detected in high caries individuals. Two Streptococcus spp. were identified only in low caries S. salivarius and S. gordonii whereas S. sanguinis, S. mitis, S. sinensis, S. rubneri, S. vestibularis, S. cristatus and S. massiliensis were identified only in individuals with high caries. This study revealed the absence of R. mucilaginosa in the high caries subjects and its coexistence with the low caries subjects. Streptococcus mutans was insignificant contributor of caries among samples, while, Streptococcus sanguinis was the main constituent of high caries Saudi patients.
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影响因子：
5.7
作者：
Rosier BT;Palazón C;García-Esteban S;Artacho A;Galiana A;Mira A
通讯作者：
Mira A
影响因子：
3.7
作者：
Sarkar A;Stoneking M;Nandineni MR
通讯作者：
Nandineni MR
影响因子：
3
作者：
Karabudak, Sema;Ari, Oguz;Durmaz, Riza
通讯作者：
Durmaz, Riza
DOI：
10.1080/20009666.2021.1880539
发表时间：
2021
影响因子：
1
作者：
Elkattawy S;Alyacoub R;Younes I;Mowafy A;Noori M;Mirza M
通讯作者：
Mirza M
影响因子：
10.7
作者：
Katoh K;Standley DM
通讯作者：
Standley DM