Transcriptomic analysis reveals key factors in fruit ripening and rubbery texture caused by 1-MCP in papaya
Transcriptomic analysis reveals key factors in fruit ripening and rubbery texture caused by 1-MCP in papaya
复制标题
转录组分析揭示了 1-MCP 引起木瓜果实成熟和橡胶质地的关键因素
DOI：
10.1186/s12870-019-1904-x
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发表时间：
2019-07
影响因子：
5.3
通讯作者：
Li Xueping
中科院分区：
文献类型：
--
作者：
Zhu Xiaoyang;Ye Lanlan;Ding Xiaochun;Gao Qiyang;Xiao Shuangling;Tan Qinqin;Huang Jiling;Chen Weixin;Li Xueping
乙烯促进果实成熟，而乙烯的无毒拮抗剂1-甲基环丙烯（1-MCP）通过抑制乙烯受体而延缓果实成熟。然而，不适当的1-MCP处理会导致果实成熟disorders.ResultsIn这项研究中，我们表明，短期1-MCP处理（400 nL·L-1，2 h）显着延迟木瓜果实成熟与正常成熟特性。然而，长期的1-MCP处理（400 nL·L-1，16 h）会导致水果的“橡胶”质地。比较转录组分析表明，共有5529个基因在果实成熟过程中与新鲜果实相比有不同的表达。综合功能富集分析表明，碳代谢、植物激素信号转导、氨基酸生物合成、淀粉和蔗糖代谢等代谢途径参与果实成熟。1-MCP处理显著影响果实转录水平。在短期1-MCP处理和长期1-MCP处理与对照之间，分别鉴定出3595和5998个差异表达基因（DEG）。DEG主要富集在与果实成熟有关的类似途径中。在长期和短期1-MCP处理之间也鉴定出大量DEG，其中大部分DEG富集在碳代谢、淀粉和蔗糖代谢、植物激素信号转导和氨基酸生物合成中。1-MCP处理促进了果实成熟过程中木质素的积累，延缓了纤维素的降解。结合番木瓜橡胶状表型，推测细胞壁代谢和激素信号通路与番木瓜果实成熟障碍密切相关。利用RT-qPCR检测了28个参与细胞壁代谢和激素信号通路的基因，证实了RNA-Seq输出结果。结论长期1-MCP处理严重抑制了乙烯信号和细胞壁代谢通路，可能导致细胞壁降解和果实软化失败。我们的研究结果揭示了多个成熟相关的事件在番木瓜果实成熟，并提供了基础，了解1-MCP处理对果实成熟和调控网络的分子机制。
BackgroundEthylene promotes fruit ripening whereas 1-methylcyclopropene (1-MCP), a non-toxic antagonist of ethylene, delays fruit ripening via the inhibition of ethylene receptor. However, unsuitable 1-MCP treatment can cause fruit ripening disorders.ResultsIn this study, we show that short-term 1-MCP treatment (400 nL•L− 1, 2 h) significantly delays papaya fruit ripening with normal ripening characteristics. However, long-term 1-MCP treatment (400 nL•L− 1, 16 h) causes a “rubbery” texture of fruit. The comparative transcriptome analysis showed that a total of 5529 genes were differently expressed during fruit ripening compared to freshly harvested fruits. Comprehensive functional enrichment analysis showed that the metabolic pathways of carbon metabolism, plant hormone signal transduction, biosynthesis of amino acids, and starch and sucrose metabolism are involved in fruit ripening. 1-MCP treatment significantly affected fruit transcript levels. A total of 3595 and 5998 differently expressed genes (DEGs) were identified between short-term 1-MCP, long-term 1-MCP treatment and the control, respectively. DEGs are mostly enriched in the similar pathway involved in fruit ripening. A large number of DEGs were also identified between long-term and short-term 1-MCP treatment, with most of the DEGs being enriched in carbon metabolism, starch and sucrose metabolism, plant hormone signal transduction, and biosynthesis of amino acids. The 1-MCP treatments accelerated the lignin accumulation and delayed cellulose degradation during fruit ripening. Considering the rubbery phenotype, we inferred that the cell wall metabolism and hormone signal pathways are closely related to papaya fruit ripening disorder. The RNA-Seq output was confirmed using RT-qPCR by 28 selected genes that were involved in cell wall metabolism and hormone signal pathways.ConclusionsThese results showed that long-term 1-MCP treatment severely inhibited ethylene signaling and the cell wall metabolism pathways, which may result in the failure of cell wall degradation and fruit softening. Our results reveal multiple ripening-associated events during papaya fruit ripening and provide a foundation for understanding the molecular mechanisms underlying 1-MCP treatment on fruit ripening and the regulatory networks.
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影响因子：
5.3
作者：
通讯作者：
--
DOI：
--
发表时间：
--
期刊：
--
影响因子：
--
作者：
F. Ziliotto;M. Begheldo;A. Rasori;C. Bonghi;P. Tonutti
通讯作者：
F. Ziliotto;M. Begheldo;A. Rasori;C. Bonghi;P. Tonutti
影响因子：
46.9
作者：
Trapnell C;Williams BA;Pertea G;Mortazavi A;Kwan G;van Baren MJ;Salzberg SL;Wold BJ;Pachter L
通讯作者：
Pachter L
影响因子：
7
作者：
Dek, Mohd Sabri Pak;Padmanabhan, Priya;Paliyath, Gopinadhan
通讯作者：
Paliyath, Gopinadhan
DOI：
--
发表时间：
1972
期刊：
Methods of biochemical analysis
影响因子：
--
作者：
A. Ermakov;V. V. Arasimovich-V.;M. I. Smirnova-Ikonnikova;N. Yarosh;G. A. Lukovnikova
通讯作者：
A. Ermakov;V. V. Arasimovich-V.;M. I. Smirnova-Ikonnikova;N. Yarosh;G. A. Lukovnikova