Delaying or delivering: identification of novel NAM-1 alleles that delay senescence to extend wheat grain fill duration.
Delaying or delivering: identification of novel NAM-1 alleles that delay senescence to extend wheat grain fill duration.
复制标题
延缓或传递：延缓衰老延长小麦籽粒灌浆期的新型NAM-1等位基因的鉴定。
DOI：
10.1093/jxb/erab368
复制
发表时间：
2021-12-04
影响因子：
6.9
通讯作者：
Griffiths S
中科院分区：
文献类型：
--
作者：
Chapman EA;Orford S;Lage J;Griffiths S
衰老是遗传和环境控制下的一个复杂性状，其中资源从营养组织重新转移到谷物中。延迟衰老或“保持绿色”特征可以赋予胁迫耐受性，延长光合活动，假设可以维持籽粒灌浆。衰老调控的遗传学在很大程度上是未知的，衰老变异通常与物候特征相关。在这里，我们确认了两种小麦品种的保持绿色表型。 Paragon 甲基磺酸乙酯突变体先前在正向遗传筛选中鉴定，并根据其农艺性能、相似的物候和差异的衰老表型进行选择。籽粒灌浆实验证实了衰老开始与籽粒灌浆持续时间之间存在正相关关系，报告称，一种突变体的最终干粒重增加约 14%（P<0.05）。为了性状作图目的而开发了针对衰老时间进行分离的重组自交系 (RIL) 群体，并在田间条件下进行了多年的表型分析。衰老指标的量化和比较有助于 RIL 选择，促进外显子组捕获的批量分离分析 (BSA)。使用 BSA，我们将两个 Staygreen 性状映射到两个独立的显性基因座，大小分别为 4.8 和 16.7 Mb，包含 56 和 142 个基因。将关联分析与变异效应预测相结合，我们鉴定了编码位于 NAM-1 同源物中的自我验证突变的单核苷酸多态性，我们将其作为候选基因。鉴定出两个保持绿色的突变体，其籽粒灌浆持续时间和籽粒重量得到增强。 NAM-1 同源物的 NAC 亚结构域 IV 内的独立氨基酸取代被认为是原因。
Senescence is a complex trait under genetic and environmental control, in which resources are remobilized from vegetative tissue into grain. Delayed senescence, or ‘staygreen’ traits, can confer stress tolerance, with extended photosynthetic activity hypothetically sustaining grain filling. The genetics of senescence regulation are largely unknown, with senescence variation often correlated with phenological traits. Here, we confirm staygreen phenotypes of two Triticum aestivum cv. Paragon ethyl methane sulfonate mutants previously identified during a forward genetic screen and selected for their agronomic performance, similar phenology, and differential senescence phenotypes. Grain filling experiments confirmed a positive relationship between onset of senescence and grain fill duration, reporting an associated ~14% increase in final dry grain weight for one mutant (P<0.05). Recombinant inbred line (RIL) populations segregating for the timing of senescence were developed for trait mapping purposes and phenotyped over multiple years under field conditions. Quantification and comparison of senescence metrics aided RIL selection, facilitating exome capture-enabled bulk segregant analysis (BSA). Using BSA we mapped our two staygreen traits to two independent, dominant, loci of 4.8 and 16.7 Mb in size encompassing 56 and 142 genes, respectively. Combining association analysis with variant effect prediction, we identified single nucleotide polymorphisms encoding self-validating mutations located in NAM-1 homoeologues, which we propose as gene candidates. Two staygreen mutants were identified for which grain fill duration and grain weight were enhanced. Independent amino acid substitutions within NAC subdomain IV of NAM-1 homoeologues are proposed as causative.
登录
查看更多内容
影响因子：
12.3
作者：
Alaux M;Rogers J;Letellier T;Flores R;Alfama F;Pommier C;Mohellibi N;Durand S;Kimmel E;Michotey C;Guerche C;Loaec M;Lainé M;Steinbach D;Choulet F;Rimbert H;Leroy P;Guilhot N;Salse J;Feuillet C;International Wheat Genome Sequencing Consortium;Paux E;Eversole K;Adam-Blondon AF;Quesneville H
通讯作者：
Quesneville H
一种还原主义的方法，用于剖析小麦的晶粒重量和产量。
影响因子：
11.4
作者：
Brinton J;Uauy C
通讯作者：
Uauy C
影响因子：
5.9
作者：
Brinton J;Ramirez-Gonzalez RH;Simmonds J;Wingen L;Orford S;Griffiths S;10 Wheat Genome Project;Haberer G;Spannagl M;Walkowiak S;Pozniak C;Uauy C
通讯作者：
Uauy C
影响因子：
4.4
作者：
Cantu D;Pearce SP;Distelfeld A;Christiansen MW;Uauy C;Akhunov E;Fahima T;Dubcovsky J
通讯作者：
Dubcovsky J
影响因子：
3.7
作者：
Alhabbar Z;Yang R;Juhasz A;Xin H;She M;Anwar M;Sultana N;Diepeveen D;Ma W;Islam S
通讯作者：
Islam S