High-throughput screening for agonists of ROS production in live human vascular endothelial cells.
High-throughput screening for agonists of ROS production in live human vascular endothelial cells.
复制标题
高通量筛选人血管内皮细胞活性氧产生的激动剂。
DOI：
10.1016/j.xpro.2021.101053
复制
发表时间：
2022-03-18
期刊：
影响因子：
--
通讯作者：
Hoshi M
中科院分区：
文献类型：
--
作者：
Sasahara T;Hoshi M
活性氧（ROS）是重要的生理分子，识别ROS产生的激动剂可以为未来的研究提供有用的工具。在这里，我们提出了一个优化的协议，高通量筛选激动剂，诱导活性氧的生产。我们描述了在人血管内皮细胞中使用荧光探针，这可以确定ROS的产生是否发生在线粒体或活细胞的质膜中。有关此配置文件的使用和执行的完整详细信息，请参阅。这是诱导ROS产生的激动剂的高通量筛选方案。通过荧光成像鉴定人内皮细胞中的ROS产生。这揭示了ROS产生是否发生在线粒体或质膜上。活性氧（ROS）是重要的生理分子，鉴定ROS产生的激动剂可以为未来的研究提供有用的工具。在这里，我们提出了一个优化的协议，高通量筛选激动剂，诱导活性氧的生产。我们描述了在人血管内皮细胞中使用荧光探针，这可以确定ROS的产生是否发生在线粒体或活细胞的质膜中。
Reactive oxygen species (ROS) are important physiological molecules, and identifying agonists for ROS production can yield useful tools for future research. Here we present an optimized protocol for high-throughput screening for agonists that induce ROS production. We describe the use of a fluorescent probe in human vascular endothelial cells, which can establish whether ROS production occurs in mitochondria or in the plasma membrane of live cells. For complete details on the use and execution of this profile, please refer to. This is a high-throughput screening protocol for agonists that induce ROS production ROS production in human endothelial cells is identified by fluorescence imaging This reveals whether ROS production occurs in mitochondria or plasma membrane Reactive oxygen species (ROS) are important physiological molecules, and identifying agonists for ROS production can yield useful tools for future research. Here we present an optimized protocol for high-throughput screening for agonists that induce ROS production. We describe the use of a fluorescent probe in human vascular endothelial cells, which can establish whether ROS production occurs in mitochondria or in the plasma membrane of live cells.
影响因子：
5.8
作者：
Sasahara T;Satomura K;Tada M;Kakita A;Hoshi M
通讯作者：
Hoshi M