Identification of a testis-enriched heat shock protein and fourteen members of Hsp70 family in the swamp eel.
Identification of a testis-enriched heat shock protein and fourteen members of Hsp70 family in the swamp eel.
复制标题
沼泽鳗睾丸富含热休克蛋白和 Hsp70 家族 14 个成员的鉴定
DOI：
10.1371/journal.pone.0065269
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Cheng H
中科院分区：
文献类型：
--
作者：
He Y;Luo M;Yi M;Sheng Y;Cheng Y;Zhou R;Cheng H
背景 性腺分化是脊椎动物最重要的发育事件之一。一些热休克蛋白与性腺发育有关。硬骨鱼中的热休克蛋白 70 (Hsp70) 及其在性别分化中的作用尚不清楚。方法和结果我们利用蛋白质印迹分析和质谱 (MS) 鉴定了沼泽鳗鱼中富含睾丸的热休克蛋白 Hspa8b2。根据转录组信息，在该物种中进一步鉴定了 14 个 Hsp70 家族基因。采用最大似然法构建Hsp70家族的系统发育树，并通过逆转录聚合酶链反应（RT-PCR）分析其在沼泽鳗性腺中的表达模式。结论 沼泽鳗基因组中Hsp70家族共有14个基因成员。 Hsp70 家族，特别是 Hspa8，在该物种中得到了扩展。 Hspa8b2 家族成员之一主要在沼泽鳗的睾丸中表达。
Background Gonad differentiation is one of the most important developmental events in vertebrates. Some heat shock proteins are associated with gonad development. Heat shock protein 70 (Hsp70) in the teleost fish and its roles in sex differentiation are poorly understood. Methods and Findings We have identified a testis-enriched heat shock protein Hspa8b2 in the swamp eel using Western blot analysis and Mass Spectrometry (MS). Fourteen Hsp70 family genes were further identified in this species based on transcriptome information. The phylogenetic tree of Hsp70 family was constructed using the Maximum Likelihood method and their expression patterns in the swamp eel gonads were analyzed by reverse transcription-polymerase chain reaction (RT-PCR). Conclusion There are fourteen gene members in the Hsp70 family in the swamp eel genome. Hsp70 family, particularly Hspa8, has expanded in the species. One of the family members Hspa8b2 is predominantly expressed in testis of the swamp eel.
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影响因子：
4.5
作者：
Lavery R;Chassot AA;Pauper E;Gregoire EP;Klopfenstein M;de Rooij DG;Mark M;Schedl A;Ghyselinck NB;Chaboissier MC
通讯作者：
Chaboissier MC
DOI：
10.1530/ror.0.0040023
发表时间：
1999-01-01
期刊：
REVIEWS OF REPRODUCTION
影响因子：
--
作者：
Eddy, EM
通讯作者：
Eddy, EM
影响因子：
2.9
作者：
Filipczak, Piotr Teodor;Piglowski, Wojciech;Scieglinska, Dorota
通讯作者：
Scieglinska, Dorota
影响因子：
2.7
作者：
Ito, Y;Ando, A;Fujimoto, H
通讯作者：
Fujimoto, H
影响因子：
8
作者：
Mayer, MP;Bukau, B
通讯作者：
Bukau, B