Production of p53 gene knockout rats by homologous recombination in embryonic stem cells.
Production of p53 gene knockout rats by homologous recombination in embryonic stem cells.
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作者：
The use of homologous recombination to modify genes in embryonic stem (ES) cells provides a powerful means to elucidate gene function and create disease models. Application of this technology to engineer genes in rats has previously been impossible in the absence of germline competent ES cells in this species. We have recently established authentic rat ES cells. Here we report the generation of the first gene knockout rats using the ES cell-based gene targeting technology. We designed a targeting vector to disrupt the tumor suppressor gene p53 (also known as Tp53) in rat ES cells via homologous recombination. p53 gene-targeted rat ES cells can be routinely generated. Furthermore, the p53 gene-targeted mutation in the rat ES cell genome can transmit through the germline via ES cell-rat chimeras to create p53 gene knockout rats. The rat is the most widely used animal model other than humans in biological research. The establishment of gene targeting technology in rat ES cells, in combination with advances in genomics and the vast amount of research data on physiology and pharmacology in this species, now provides a powerful new platform for the study of human disease.
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影响因子：
64.8
作者：
Ying, Qi-Long;Wray, Jason;Smith, Austin
通讯作者：
Smith, Austin
影响因子：
64.5
作者：
Li P;Tong C;Mehrian-Shai R;Jia L;Wu N;Yan Y;Maxson RE;Schulze EN;Song H;Hsieh CL;Pera MF;Ying QL
通讯作者：
Ying QL
影响因子：
64.5
作者：
Ying, QL;Nichols, J;Smith, A
通讯作者：
Smith, A
影响因子：
30.8
作者：
Aitman, Timothy J.;Critser, John K.;Worley, Kim
通讯作者：
Worley, Kim
DOI：
10.1126/science.1172447
发表时间：
2009-07-24
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Geurts AM;Cost GJ;Freyvert Y;Zeitler B;Miller JC;Choi VM;Jenkins SS;Wood A;Cui X;Meng X;Vincent A;Lam S;Michalkiewicz M;Schilling R;Foeckler J;Kalloway S;Weiler H;Ménoret S;Anegon I;Davis GD;Zhang L;Rebar EJ;Gregory PD;Urnov FD;Jacob HJ;Buelow R
通讯作者：
Buelow R