A separation-of-function ZIP4 wheat mutant allows crossover between related chromosomes and is meiotically stable.
A separation-of-function ZIP4 wheat mutant allows crossover between related chromosomes and is meiotically stable.
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DOI：
10.1038/s41598-021-01379-z
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发表时间：
2021-11-08
影响因子：
4.6
通讯作者：
Moore G
中科院分区：
文献类型：
--
作者：
Martín AC;Alabdullah AK;Moore G
许多物种，包括大多数开花植物，都是多倍体，拥有多个基因组。在多倍化过程中，通过控制这些多基因组在减数分裂过程中的行为的机制的进化来保持生育力。在小麦多倍化过程中，减数分裂主效基因ZIP 4发生了复制和分化，产生的新基因TaZIP 4-B2插入到5 B染色体上。先前的研究表明，TaZIP 4-B2促进小麦同源染色体之间的配对和联会，同时抑制相关（同源）染色体之间的交换。此外，在小麦中，TaZIP 4-B2的存在保留了高达50%的谷粒数量。本研究利用了一种名为zip 4-ph 1d的“功能分离”小麦Tazip 4-B2突变体，其中Tazip 4-B2拷贝仍然促进同源物之间的正确配对和突触（导致与野生型小麦中通常发现的相同的花粉特征和生育力），但也允许该突变体的小麦单倍体中相关染色体之间的交叉。这表明，与先前描述的CRISPR Tazip 4-B2和ph 1突变系相比，新的zip 4-ph 1d突变系在小麦育种期间的效用有所改善。结果还表明，当TaZIP 4-B2促进同源配对和突触被保留时，小麦染色体之间同源异源交换抑制的丧失本身并不降低小麦育性。
Many species, including most flowering plants, are polyploid, possessing multiple genomes. During polyploidisation, fertility is preserved via the evolution of mechanisms to control the behaviour of these multiple genomes during meiosis. On the polyploidisation of wheat, the major meiotic gene ZIP4 duplicated and diverged, with the resulting new gene TaZIP4-B2 being inserted into chromosome 5B. Previous studies showed that this TaZIP4-B2 promotes pairing and synapsis between wheat homologous chromosomes, whilst suppressing crossover between related (homoeologous) chromosomes. Moreover, in wheat, the presence of TaZIP4-B2 preserves up to 50% of grain number. The present study exploits a ‘separation-of-function’ wheat Tazip4-B2 mutant named zip4-ph1d, in which the Tazip4-B2 copy still promotes correct pairing and synapsis between homologues (resulting in the same pollen profile and fertility normally found in wild type wheat), but which also allows crossover between the related chromosomes in wheat haploids of this mutant. This suggests an improved utility for the new zip4-ph1d mutant line during wheat breeding, compared to the previously described CRISPR Tazip4-B2 and ph1 mutant lines. The results also reveal that loss of suppression of homoeologous crossover between wheat chromosomes does not in itself reduce wheat fertility when promotion of homologous pairing and synapsis by TaZIP4-B2 is preserved.
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DOI：
10.1073/pnas.0801521105
发表时间：
2008-04-22
影响因子：
11.1
作者：
Colas, Isabelle;Shaw, Peter;Moore, Graham
通讯作者：
Moore, Graham
影响因子：
5.4
作者：
JAUHAR, PP;RIERALIZARAZU, O;BENNETT, JH
通讯作者：
BENNETT, JH
影响因子：
16.6
作者：
Martín AC;Shaw P;Phillips D;Reader S;Moore G
通讯作者：
Moore G
影响因子：
14.9
作者：
Drozdetskiy A;Cole C;Procter J;Barton GJ
通讯作者：
Barton GJ
影响因子：
5.8
作者：
Larkin, M. A.;Blackshields, G.;Higgins, D. G.
通讯作者：
Higgins, D. G.