Characterization and early embryonic expression of a neural specific transcription factor xSOX3 in Xenopus laevis.
Characterization and early embryonic expression of a neural specific transcription factor xSOX3 in Xenopus laevis.
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非洲爪蟾神经特异性转录因子 xSOX3 的表征和早期胚胎表达。
DOI：
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发表时间：
1997
影响因子：
0.7
通讯作者：
H. Grunz
中科院分区：
文献类型：
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作者：
R. Penzel;R. Oschwald;Y. Chen;L. Tacke;H. Grunz
利用强大的rda - pcr技术，我们鉴定出了一个新的爪蟾特异性sox基因（xSox3），这是一个与sox亚群B密切相关的转录因子，它包含一个HMG盒子。在正常发育中，xSox3基因在假定的中枢神经系统中表达。此外，在动物半球卵裂早期的卵母细胞中也发现了母系成分。通过全载原位杂交，可以检测到外胚层后部囊胚和边缘区后部及外侧囊胚的第一次合子转录活性。在原肠晚期至神经晚期表达最高，30期后逐渐消失。从原肠胚开始，这种表达仅限于推定的脑区和晶状体上皮。此外，我们可以证明该基因在与相邻神经外胚层分离的精子组织中表达。苏拉明治疗可抑制该信号，从而抑制神经发育并导致中胚层从背侧向腹侧转移。用LiCl和UV处理全胚分别导致过表达和抑制表达。在外泌乳（假外泌乳）中，该基因仅在内胚层附近表达，而在外胚层的远端大部分不表达。这一结果表明，该基因不太可能被平面信号激活。该基因也可以在游离的原胚外胚层中被激活，而不需要中胚层或神经诱导剂。这意味着该基因可以在外胚层细胞中以细胞自主的方式表达。
Using the powerful RDA-PCR-technique we could identify a novel Xenopus specific Sox-gene (xSox3) a transcription factor closely related to the sox sub-group B, which contains a HMG box. In normogenesis the xSox3 gene is expressed in the presumptive central nervous system. Furthermore a maternal component is also found in oocytes and in early cleavage stages in the animal hemisphere only. By whole-mount in situ hybridization the first zygotic transcription activities can be detected in the late blastula in the dorsal ectoderm and the dorsal and lateral part of the marginal zone. The expression reaches the highest level atthe late gastrula till the late neurula and fades after stage 30. The expression is restricted from gastrulation onwards to the presumptive brain area and the lens epithelium. Furthermore we could show that the gene is expressed in isolated Spemann organizer with adjacent neuroectoderm. The signal can be suppressed by suramin treatment, which inhibits neural development and causes a shift of dorsal to ventral mesoderm. The treatment of whole embryos with LiCl and UV results in an overexpression or an inhibition of the expression, respectively. In exogastrulae (pseudo-exogastrulae) the gene is expressed in the close vicinity to the endomesoderm only, but not in the distal most part of the ectoderm. This result indicates that it is unlikely that the gene can be activated by planar signals. The gene can also be activated in dissociated gastrula ectoderm without mesodermal or neural inducers. That means that the gene can be expressed in ectodermal cells in a cell autonomous manner.
含 HMG 盒基因的 Sox 家族的许多成员在发育中的小鼠牙齿中表达。
DOI：
10.1006/geno.1996.0548
发表时间：
1996
期刊：
Genomics.
影响因子：
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作者：
Stock,DW;Buchanan,AV;Zhao,Z;Weiss,KM
通讯作者：
Weiss,KM