Amelioration of Cigarette Smoke-Induced Mucus Hypersecretion and Viscosity by Dendrobium officinale Polysaccharides In Vitro and In Vivo.
Amelioration of Cigarette Smoke-Induced Mucus Hypersecretion and Viscosity by Dendrobium officinale Polysaccharides In Vitro and In Vivo.
复制标题
DOI：
10.1155/2020/8217642
复制
发表时间：
2020
影响因子：
--
通讯作者：
Mak JCW
中科院分区：
文献类型：
--
作者：
Chen R;Liang Y;Ip MSM;Zhang KY;Mak JCW
以氧化应激和炎症为特征的慢性阻塞性肺疾病（COPD）是世界范围内的主要死亡原因之一，其中香烟烟雾（CS）是主要危险因素。铁皮石斛多糖（Dendrobium officinale polysaccharides，DOP）是从铁皮石斛中提取的主要活性成分，具有抗氧化、抗炎、抑制粘蛋白基因表达等作用。本研究旨在研究DOP对CS诱导的粘液高分泌和粘度的体外和体内影响。对于体外研究，在不存在或存在各种浓度的DOP或N-乙酰半胱氨酸（NAC）的情况下，用香烟烟雾培养基（CSM）刺激在空气-液体界面（ALI）培养28天的原代正常人支气管上皮细胞（HBEC）24小时。在体内研究中，将雄性Sprague-Dawley大鼠随机分为假空气（SA）对照组和CS组，共56天。在第29天，将大鼠细分，并在剩余的持续时间内通过口服管饲法给予水作为对照，给予DOP或NAC作为阳性对照作为粘液溶解药物。评价从心尖冲洗液、细胞裂解物、支气管肺泡灌洗（BAL）和肺组织采集的样本的粘蛋白基因表达、粘液分泌和粘度。DOPs可通过抑制粘液分泌颗粒而下调MUC 5AC mRNA、MUC 5AC分泌蛋白和粘液粘度，从而改善CS诱导的粘液高分泌和粘度。DOPs通过抑制粘液分泌颗粒而对CS诱导的粘液高分泌和粘液粘度产生影响。这些发现可能是考虑DOP在吸烟介导的COPD管理中的潜在作用的起点。然而，还需要进一步的研究。
Chronic obstructive pulmonary disease (COPD), characterized by oxidative stress and inflammation, is one of the leading causes of death worldwide, in which cigarette smoke (CS) is the major risk factor. Dendrobium officinale polysaccharides (DOPs) are the main active ingredients extracted from Dendrobium officinale, which have been reported to have antioxidant and anti-inflammatory activity as well as inhibition of mucin gene expression. This study is aimed at investigating the effect of DOPs on CS-induced mucus hypersecretion and viscosity in vitro and in vivo. For in vitro study, primary normal human bronchial epithelial cells (HBECs) differentiated at the air-liquid interface (ALI) culture for 28 days were stimulated with cigarette smoke medium (CSM) in the absence or presence of various concentrations of DOPs or N-acetylcysteine (NAC) for 24 hours. For in vivo study, male Sprague-Dawley rats were randomized to sham air (SA) as control group or CS group for 56 days. At day 29, rats were subdivided and given water as control, DOPs, or NAC as positive control as a mucolytic drug via oral gavage for the remaining duration. Samples collected from apical washing, cell lysates, bronchoalveolar lavage (BAL), and lung tissues were evaluated for mucin gene expression, mucus secretion, and viscosity. DOPs ameliorated the CS-induced mucus hypersecretion and viscosity as shown by the downregulation of MUC5AC mRNA, MUC5AC secretary protein, and mucus viscosity via inhibition of mucus secretory granules in both in vitro and in vivo models. DOPs produced its effective effects on the CS-induced mucus hypersecretion and viscosity via the inhibition of the mucus secretory granules. These findings could be a starting point for considering the potential role of DOPs in the management of the smoking-mediated COPD. However, further research is needed.
登录
查看更多内容
DOI：
10.1155/2015/841752
发表时间：
2015
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Lam Y;Ng TB;Yao RM;Shi J;Xu K;Sze SC;Zhang KY
通讯作者：
Zhang KY
DOI：
10.1056/nejmra0910061
发表时间：
2010-12-02
期刊：
The New England journal of medicine
影响因子：
--
作者：
Fahy JV;Dickey BF
通讯作者：
Dickey BF
影响因子：
5.6
作者：
Liang Y;Liu KWK;Yeung SC;Li X;Ip MSM;Mak JCW
通讯作者：
Mak JCW
影响因子：
6.4
作者：
Caramori, G;Di Gregorio, C;Papi, A
通讯作者：
Papi, A
影响因子：
6.4
作者：
Caramori, Gaetano;Casolari, Paolo;Papi, Alberto
通讯作者：
Papi, Alberto