Changes in motility that accompany the acrosome reaction in hyperactivated hamster spermatozoa
Changes in motility that accompany the acrosome reaction in hyperactivated hamster spermatozoa
复制标题
过度活跃的仓鼠精子顶体反应伴随的运动变化
DOI：
10.1002/mrd.1120100305
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
S. Meizel
中科院分区：
文献类型：
--
作者：
S. Suarez;D. Katz;S. Meizel
用高速摄像显微镜对体外完成顶体反应的仓鼠附睾精子和同一样本中未反应精子的运动特征进行了比较。在含牛血清白蛋白、牛磺酸和肾上腺素的改良台氏培养基中孵育4.25小时后，90%以上的活动精子被过度激活，约一半的精子发生顶体反应。与未反应精子相比，反应精子的鞭毛跳动频率明显降低，弯曲程度更明显。较低的节拍频率并不归因于老化，因为精子诱导反应同步在3.5小时使用溶血磷脂酰胆碱节拍与类似的频率降低。顶体反应和未反应的超活化精子游泳的圆形轨迹造成不对称的鞭毛跳动。未反应的精子的鞭毛跳动更加对称;因此，它们以更大的圆圈游泳，并有可能更快地覆盖空间。一些未反应的精子，也许在过渡到过度活化，在螺旋轨道游泳。
当将预孵育的精子加入到含有卵丘中卵母细胞的载玻片中时，一些未反应的精子开始穿透卵丘。所有反应的精子都没有这样做，而是粘附在其边界的积云上。反应精子通过顶体内膜区附着于无卵丘卵母细胞的透明带。未反应的精子通过赤道区附着，但围绕附着点旋转，因此无法产生持续的推力。总之，未反应的超活化精子与反应的精子在运动和与卵母细胞相互作用方面具有不同的潜力。
High-speed videomicrography was used to compare the movement characteristics of hamster epididymal sperm which completed the acrosome reaction in vitro with those of unreacted sperm in the same sample. More than 90% of the motile sperm incubated for 4.25 hr in a modified Tyrode's medium containing bovine serum albumin, taurine, and epinephrine were hyperactivated and about half were acrosome reacted. The flagella of reacted sperm beat with significantly lower frequency and bent into more acute curves than those of unreacted sperm. Lowered beat frequencies were not attributable to aging, because sperm induced to react synchronously at 3.5 hr using lysophosphatidyl choline beat with similar lowered frequencies. Both acrosome-reacted and unreacted hyperactivated sperm swam in circular trajectories resulting from asymmetrical flagellar beating. The flagellar beating of unreacted sperm was more symmetrical; consequently, they swam in larger circles and had the potential to cover space more rapidly. Some unreacted sperm, perhaps in transition towards hyperactivation, swam in helical trajectories.
When preincubated sperm were added to slides containing oocytes in cumulus, some unreacted sperm initiated cumulus penetration. All reacted sperm failed to do so, adhering instead to the cumulus at its boundary. Reacted sperm attached to the zonae pellucidae of cumulus-free oocytes via the region of the inner acrosomal membrane. Unreacted sperm attached via the equatorial region, but pivoted about the point of attachment, thus failing to generate sustained thrust against the zona. In conclusion, unreacted hyperactivated sperm have a different potential than reacted sperm for movement and interaction with egg vestments.
仓鼠和豚鼠精子附着于透明带后的运动特征。
影响因子：
3.6
作者：
Katz,DF;Yanagimachi,R
通讯作者：
Yanagimachi,R
DOI：
10.1530/jrf.0.0630509
发表时间：
1981-11
期刊：
Journal of reproduction and fertility
影响因子：
--
作者：
A. D. Fleming;R. Yanagimachi;H. Yanagimachi
通讯作者：
A. D. Fleming;R. Yanagimachi;H. Yanagimachi