Pressure-Spun Fibrous Surgical Sutures for Localized Antibacterial Delivery: Development, Characterization, and In Vitro Evaluation.
Pressure-Spun Fibrous Surgical Sutures for Localized Antibacterial Delivery: Development, Characterization, and In Vitro Evaluation.
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DOI：
10.1021/acsami.3c07956
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发表时间：
2023-10-04
影响因子：
9.5
通讯作者：
Edirisinghe, Mohan
中科院分区：
文献类型：
--
作者：
Altun, Esra;Bayram, Cem;Gultekinoglu, Merve;Matharu, Rupy;Delbusso, Angelo;Homer-Vanniasinkam, Shervanthi;Edirisinghe, Mohan
为防止感染而设计的手术缝线在解决导致手术部位感染的抗生素耐药病原体方面至关重要。由于全球新出现的抗生素耐药性问题，已对杀菌剂等替代材料用于覆盖商业使用的缝合线进行了评估。这项研究为开发具有局部抗菌药物输送能力的纤维外科缝合线提供了一种新的方法。首次将抗菌三氯生(Tri)与聚乳酸-乙醇酸共聚(PLGA)和聚环氧乙烷(PEO)聚合物物理共混，采用压力纺丝法生产纤维外科缝合线。采用原生PLGA、原生PEO、不同比例的PLGA-PEO和不同比例的三载型PLGA-PEO纤维缝合线，模拟FDA和NICE批准的基于PLGA的缝合线，并比较它们的特点。同时用商业缝合线进行测试，比较它们的体外生物降解性、抗菌性、药物释放和细胞毒性。体外抗菌试验24 h后，选择2 85±12μg/mgTri负载量的缝合线作为进一步试验的模型，因为它们对所有受试菌株都具有抗菌活性。选定的抗菌纤维缝线模型在24小时内表现出Tri的初始释放，随后在剩余时间内持续释放，直到缝合线在21天内完全降解。细胞活性测定表明，这些手术缝线对哺乳动物细胞无细胞毒性作用。
Surgical sutures designed to prevent infection are critical in addressing antibiotic-resistant pathogens that cause surgical site infections. Instead of antibiotics, alternative materials such as biocides have been assessed for coating commercially used sutures due to emerging antibiotic resistance concerns worldwide. This study has a new approach to the development of fibrous surgical sutures with the ability to deliver localized antibacterial agents. A new manufacturing process based on pressure spinning was used for the first time in the production of fibrous surgical sutures by physically blending antibacterial triclosan (Tri) agent with poly(lactic-co-glycolic acid) (PLGA) and poly(ethylene oxide) (PEO) polymers. Fibrous surgical sutures with virgin PLGA, virgin PEO, different ratios of PLGA–PEO, and different ratios of Tri-loaded PLGA–PEO fibrous sutures were produced to mimic the FDA- and NICE-approved PLGA-based sutures available in the market and compared for their characteristics. They were also tested simultaneously with commercially available sutures to compare their in vitro biodegradation, antibacterial, drug release, and cytotoxicity properties. After in vitro antibacterial testing for 24 h, sutures having 285 ± 12 μg/mg Tri loading were selected as a model for further testing as they exhibited antibacterial activity against all tested bacteria strains. The selected model of antibacterial fibrous sutures exhibited an initial burst of Tri release within 24 h, followed by a sustained release for the remaining time until the sutures completely degraded within 21 days. The cell viability assay showed that these surgical sutures had no cytotoxic effect on mammalian cells.
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影响因子：
3.9
作者：
Altun, Esra;Aydogdu, Mehmet Onur;Edirisinghe, Mohan
通讯作者：
Edirisinghe, Mohan
影响因子：
9.5
作者：
Bae, Sooneon;DiBalsi, Michael J.;Lee, Jeoung Soo
通讯作者：
Lee, Jeoung Soo
影响因子：
6
作者：
Altun, Esra;Ahmed, Jubair;Edirisinghe, Mohan
通讯作者：
Edirisinghe, Mohan
DOI：
10.1002/jbm.820170612
发表时间：
1983-01-01
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
CHU, CC;WILLIAMS, DF
通讯作者：
WILLIAMS, DF
影响因子：
3.4
作者：
Eroglu, Ipek;Gultekinoglu, Merve;Ulubayram, Kezban
通讯作者：
Ulubayram, Kezban