Loop-Mediated Isothermal Amplification Methods for Diagnosis of Bacterial Meningitis.
Loop-Mediated Isothermal Amplification Methods for Diagnosis of Bacterial Meningitis.
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DOI：
10.3389/fped.2018.00057
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发表时间：
2018
影响因子：
2.6
通讯作者：
Kim DW
中科院分区：
文献类型：
--
作者：
Seki M;Kilgore PE;Kim EJ;Ohnishi M;Hayakawa S;Kim DW
快速、准确和有效地识别传染病对于确保公共卫生环境中及时的临床治疗和预防至关重要。2015年，由肺炎链球菌、流感嗜血杆菌和脑膜炎奈瑟菌引起的脑膜炎导致379,200人(范围：322,700-444,700人)死亡。仅凭临床特征不能确定是否存在细菌性脑膜炎；脑脊液(CSF)的分析是必不可少的。环介导的等温扩增(LAMP)是一种核酸扩增方法，提供了一种替代聚合酶链式反应(PCR)的方法。建立了基于LAMP的脑脊液中三种主要细菌的检测方法，用于诊断脑膜炎。开展了用LAMP检测A、B、C、W、X、Y群脑膜炎双球菌和流感嗜血杆菌a、b、c、d、e、f型的分型试验。在脑膜炎病原菌检测的对比分析中，LAMP检测无假阴性结果，且LAMP检测的检出率优于PCR法或常规培养法。LAMP检测提供准确和快速的检测结果，以检测主要的细菌性脑膜炎病原体。越来越多的证据表明，在发达国家和发展中国家资源有限的情况下，LAMP检测有可能为细菌性脑膜炎提供急需的诊断。
The rapid, accurate, and efficient identification of an infectious disease is critical to ensure timely clinical treatment and prevention in public health settings. In 2015, meningitis caused by Streptococcus pneumoniae, Haemophilus influenzae, and Neisseria meningitidis was responsible for 379,200 (range: 322,700–444,700) deaths. Clinical features alone cannot determine whether bacterial meningitis is present; an analysis of cerebrospinal fluid (CSF) is essential. Loop-mediated isothermal amplification (LAMP) is a nucleic acid amplification method offering an alternative to polymerase chain reaction (PCR). LAMP-based assays for detection of three leading bacteria in CSF for diagnosis of meningitis have been established. The typing assays using LAMP for detection of meningococcal serogroups A, B, C, W, X, and Y as well as H. influenzae serotypes a, b, c, d, e, and f were launched. In comparative analysis of the meningitis pathogen assays, LAMP assays did not yield false negative results, and the detection rate of LAMP assays was superior compared with PCR or conventional culture methods. LAMP assays provide accurate and rapid test results to detect major bacterial meningitis pathogens. Accumulating evidence suggests that LAMP assays have the potential to provide urgently needed diagnostics for bacterial meningitis in resource-limited settings of both developed and developing countries.
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影响因子：
3.7
作者：
Messaoudi M;Milenkov M;Albrich WC;van der Linden MP;Bénet T;Chou M;Sylla M;Barreto Costa P;Richard N;Klugman KP;Endtz HP;Paranhos-Baccalà G;Telles JN
通讯作者：
Telles JN
影响因子：
2.9
作者：
Miyamoto, Shigehiko;Sano, Sotaro;Jikihara, Takaaki
通讯作者：
Jikihara, Takaaki
影响因子：
3.4
作者：
KHAN, G;KANGRO, HO;HEATH, RB
通讯作者：
HEATH, RB
影响因子：
--
作者：
Kaneko, Hisatoshi;Kawana, Takashi;Suzutani, Tatsuo
通讯作者：
Suzutani, Tatsuo
影响因子：
5.5
作者：
Kim, JS;Jang, YT;Ward, JI
通讯作者：
Ward, JI