Nucleotide sequence and exon-intron organization of the human proacrosin gene.
Nucleotide sequence and exon-intron organization of the human proacrosin gene.
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人类顶体蛋白原基因的核苷酸序列和外显子-内含子组织。
DOI：
10.1111/j.1432-1033.1990.tb15564.x
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
W. Engel
中科院分区：
文献类型：
--
作者：
S. Keime;I. Adham;W. Engel
顶体酶是一种丝氨酸蛋白酶，以酶原形式存在于精子顶体中。从人和猪顶体酶原的cDNA序列推断，该酶被合成为前酶原，前顶体酶原，其含有疏水前导序列。以cDNA克隆为探针，我们分别从人白细胞基因组文库和人粘粒文库中分离到人顶体酶原基因。该基因含有4个内含子，长度在0.2 kb-4.5 kb之间。与其他丝氨酸蛋白酶类似，前顶体蛋白原基因的编码序列分布在该基因的所有五个外显子上，并且三个活性位点残基His、Asp和Ser由三个不同的外显子编码。根据外显子-内含子结构，前顶体蛋白原被认为与丝氨酸蛋白酶亚家族（包括胰蛋白酶和激肽释放酶）密切相关。然而，顶体酶原的轻链似乎与胰凝乳蛋白酶的轻链相似。丝氨酸活性位点残基的编码连同前顶体蛋白特异性富含脯氨酸的结构域在一个外显子中，即外显子E5，让我们假设富含脯氨酸的结构域的核苷酸序列是在进化过程中通过内含子-外显子转移从外源基因产生的，随后内含子切除。通过引物延伸分析，前顶体素原mRNA的转录起始位点可定位在翻译起始密码子ATG上游-74个核苷酸的C残基上。与大多数其他真核基因（包括已知的睾丸特异性基因）不同，在顶体蛋白原基因中不能检测到距转录起始位点5'端常规距离的典型TATA和CAAT盒序列。
Acrosin is a serine proteinase and located in a zymogen form, proacrosin, in the acrosome of the sperm. As deduced from the cDNA sequences for human and boar proacrosin, the enzyme is synthesized as a preproenzyme, preproacrosin, which contains a hydrophobic leader sequence. Using cDNA clones as probes, we have isolated the gene coding for human proacrosin from a human leucocyte genomic library and a human cosmid library, respectively. The gene contains four introns between 0.2 kb--4.5 kb in length. Similar to other serine proteinases, the coding sequence of the preproacrosin gene is spread over all the five exons of the gene and the three activesite residues His, Asp and Ser are encoded by three different exons. According to the exon-intron structure, preproacrosin is suggested to be closely related to the serine proteinase subfamily containing trypsin and kallikrein. However, the light chain of proacrosin seems to be similar to that of chymotrypsin. The coding of the serine active-site residue together with the proacrosin-specific proline-rich domain in one exon, namely exon E5, let us assume that the nucleotide sequence for the proline-rich domain was generated during evolution by intron-exon transfer from a foreign gene with subsequent intron excision. By primer extension analysis, the transcription initiation site of the preproacrosin mRNA could be assigned to the residue C at -74 nucleotides upstream from the translation initiation codon ATG. In contrast to most other eucaryotic genes, including the known testis-specific genes, typical TATA and CAAT box sequences in convential distances from the 5' end of the transcription start site could not be evaluated in the proacrosin gene.
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编码人和牛纤溶酶原的互补脱氧核糖核酸的表征。
影响因子：
2.9
作者：
Malinowski,DP;Sadler,JE;Davie,EW
通讯作者：
Davie,EW
DOI：
--
发表时间：
1984-11
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
C. Craik;Q. L. Choo;G. Swift;C. Quinto;Raymond J MacDonald;W. Rutter
通讯作者：
C. Craik;Q. L. Choo;G. Swift;C. Quinto;Raymond J MacDonald;W. Rutter
DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Krawetz,SA;Connor,W;Dixon,GH
通讯作者：
Dixon,GH
影响因子：
2.9
作者：
DEGEN, SJF;DAVIE, EW
通讯作者：
DAVIE, EW
睾丸特异性 H1 组蛋白变体 H1t 基因的分离。
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Cole,KD;Kandala,JC;Kistler,WS
通讯作者：
Kistler,WS