Proteomic analysis of mesenchymal stem cells from normal and deep carious dental pulp.
Proteomic analysis of mesenchymal stem cells from normal and deep carious dental pulp.
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正常牙髓和深层龋齿牙髓间充质干细胞的蛋白质组学分析
DOI：
10.1371/journal.pone.0097026
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Wu B
中科院分区：
文献类型：
--
作者：
Ma D;Cui L;Gao J;Yan W;Liu Y;Xu S;Wu B
牙髓干细胞（DPSCs）是成牙本质细胞的前体细胞，是牙齿组织工程和再生的理想种子细胞。我们前期的研究已经证实，在深龋牙髓中存在干细胞，称为龋性牙髓干细胞（carious dental pulp stem cells，CDPSC）。结果表明，CDPSC比DPSCs具有更高的增殖能力和更强的成骨分化潜能。然而，对DPSC和CDPSC之间生物学差异的分子机制知之甚少。本研究的目的是确定DPSCs和CDPSC的分子特征，通过比较蛋白质组学的配置文件，使用二维荧光差异凝胶电泳（2-D DIGE）结合基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）。结果表明，在pH 4 ~ 7的范围内，DPSCs和CDPSC之间存在18个蛋白质点的差异表达。这些差异表达的蛋白质主要参与细胞增殖、分化、细胞骨架和运动的调节。此外，我们的研究结果表明，CDPSC具有较高的抗氧化蛋白的表达，可能保护CDPSC免受氧化应激。本研究探讨了导致DPSCs和CDPSC之间生物学差异的一些潜在蛋白质，并扩展了我们对DPSCs在牙本质-牙髓复合物形成中矿化的分子机制的理解。
Dental pulp stem cells (DPSCs), precursor cells of odontoblasts, are ideal seed cells for tooth tissue engineering and regeneration. Our previous study has demonstrated that stem cells exist in dental pulp with deep caries and are called carious dental pulp stem cells (CDPSCs). The results indicated that CDPSCs had a higher proliferative and stronger osteogenic differentiation potential than DPSCs. However, the molecular mechanisms responsible for the biological differences between DPSCs and CDPSCs are poorly understood. The aim of this study was to define the molecular features of DPSCs and CDPSCs by comparing the proteomic profiles using two-dimensional fluorescence difference gel electrophoresis (2-D DIGE) in combination with matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Our results revealed that there were 18 protein spots differentially expressed between DPSCs and CDPSCs in a narrow pH range of 4 to 7. These differently expressed proteins are mostly involved in the regulation of cell proliferation, differentiation, cell cytoskeleton and motility. In addition, our results suggested that CDPSCs had a higher expression of antioxidative proteins that might protect CDPSCs from oxidative stress. This study explores some potential proteins responsible for the biological differences between DPSCs and CDPSCs and expands our understanding on the molecular mechanisms of mineralization of DPSCs in the formation of the dentin-pulp complex.
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影响因子：
4.4
作者：
Celebi, Betul;Elcin, A. Eser;Elcin, Y. Murat
通讯作者：
Elcin, Y. Murat
影响因子：
3.7
作者：
Bronckaers A;Hilkens P;Fanton Y;Struys T;Gervois P;Politis C;Martens W;Lambrichts I
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Lambrichts I
影响因子：
3.3
作者：
Haunhorst P;Hanschmann EM;Bräutigam L;Stehling O;Hoffmann B;Mühlenhoff U;Lill R;Berndt C;Lillig CH
通讯作者：
Lillig CH
影响因子：
4.8
作者：
Banki, K;Hutter, E;Perl, A
通讯作者：
Perl, A
影响因子：
2.9
作者：
HUANG, YC;COLMAN, RF
通讯作者：
COLMAN, RF