Preparation of Extracellular Matrix Protein Fibers for Brillouin Spectroscopy.
Preparation of Extracellular Matrix Protein Fibers for Brillouin Spectroscopy.
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DOI：
10.3791/54648
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发表时间：
2016-09-15
期刊：
影响因子：
--
通讯作者：
Palombo F
中科院分区：
文献类型：
--
作者：
Edginton RS;Mattana S;Caponi S;Fioretto D;Green E;Winlove CP;Palombo F
布里渊光谱技术是生物医学领域的一项新兴技术。它通过可见光与以几公里/秒的速度传播的热致声波或声子的相互作用来探测样品的机械特性。以非破坏性的非接触方式获得关于材料的弹性和结构的信息，从而为体内应用和潜在的病理诊断开辟了道路。这项工作描述了应用布里渊光谱的弹性蛋白和胰蛋白酶消化的I型胶原纤维的细胞外基质的生物力学研究。细胞外基质的纤维蛋白是生物组织的基石，研究它们的机械和物理行为是建立正常组织和疾病中发生的变化的结构-功能关系的关键。本文介绍了用反射基片测量布里渊光谱的样品制备过程、光学系统和光谱数据分析方法。
Brillouin spectroscopy is an emerging technique in the biomedical field. It probes the mechanical properties of a sample through the interaction of visible light with thermally induced acoustic waves or phonons propagating at a speed of a few km/sec. Information on the elasticity and structure of the material is obtained in a nondestructive contactless manner, hence opening the way to in vivo applications and potential diagnosis of pathology. This work describes the application of Brillouin spectroscopy to the study of biomechanics in elastin and trypsin-digested type I collagen fibers of the extracellular matrix. Fibrous proteins of the extracellular matrix are the building blocks of biological tissues and investigating their mechanical and physical behavior is key to establishing structure-function relationships in normal tissues and the changes which occur in disease. The procedures of sample preparation followed by measurement of Brillouin spectra using a reflective substrate are presented together with details of the optical system and methods of spectral data analysis.
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