Both proliferation and lipogenesis of brown adipocytes contribute to postnatal brown adipose tissue growth in mice.
Both proliferation and lipogenesis of brown adipocytes contribute to postnatal brown adipose tissue growth in mice.
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DOI：
10.1038/s41598-020-77362-x
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发表时间：
2020-11-23
影响因子：
4.6
通讯作者：
Lin Z
中科院分区：
文献类型：
--
作者：
Negron SG;Ercan-Sencicek AG;Freed J;Walters M;Lin Z
棕色脂肪组织（BAT）是哺乳动物主要的非颤抖产热器官，在维持婴儿体温方面发挥着重要作用。尽管啮齿类动物胚胎发生过程中 BAT 的发育已得到很好的解决，但 BAT 在出生后如何生长仍不清楚。我们以小鼠肩胛间 BAT (iBAT) 为例，研究了调节出生后 BAT 生长的细胞和分子机制。通过分析棕色脂肪细胞（BA）的发育动态，我们发现 BA 尺寸增大部分是 iBAT 生长的原因。通过研究 BAs 细胞周期活动，我们证实新生小鼠中存在增殖性 BAs。出生后两周，大部分BAs退出细胞周期，BAT的进一步扩张主要是由于脂肪生成介导的BAs体积增加。显微镜和荧光激活细胞分选分析表明大多数 BA 是单核和二倍体。基于棕色脂肪细胞的发育动力学，我们提出小鼠iBAT在出生和断奶之间有两个不同的生长阶段：前两周BA大小和数量增加，此后BA大小增大。总之，我们的数据表明 BA 的脂肪生成和增殖都有助于小鼠出生后 iBAT 的生长。
Brown adipose tissue (BAT) is the primary non-shivering thermogenesis organ in mammals, which plays essential roles in maintaining the body temperature of infants. Although the development of BAT during embryogenesis has been well addressed in rodents, how BAT grows after birth remains unknown. Using mouse interscapular BAT (iBAT) as an example, we studied the cellular and molecular mechanisms that regulate postnatal BAT growth. By analyzing the developmental dynamics of brown adipocytes (BAs), we found that BAs size enlargement partially accounts for iBAT growth. By investigating the BAs cell cycle activities, we confirmed the presence of proliferative BAs in the neonatal mice. Two weeks after birth, most of the BAs exit cell cycle, and the further expansion of the BAT was mainly due to lipogenesis-mediated BAs volume increase. Microscopy and fluorescence-activated cell sorting analyses suggest that most BAs are mononuclear and diploid. Based on the developmental dynamics of brown adipocytes, we propose that the murine iBAT has two different growth phases between birth and weaning: increase of BAs size and number in the first two weeks, and BAs size enlargement thereafter. In summary, our data demonstrate that both lipogenesis and proliferation of BAs contribute to postnatal iBAT growth in mice.
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影响因子：
16.6
作者：
通讯作者：
--
DOI：
10.4137/cmc.s17067
发表时间：
2014
期刊：
Clinical Medicine Insights. Cardiology
影响因子：
--
作者：
Furuhashi M;Saitoh S;Shimamoto K;Miura T
通讯作者：
Miura T
影响因子：
--
作者：
GELOEN, A;COLLET, AJ;BUKOWIECKI, LJ
通讯作者：
BUKOWIECKI, LJ
影响因子：
2.3
作者：
Deng, Jie;Schoeneman, Samantha E.;Josefson, Jami L.
通讯作者：
Josefson, Jami L.
影响因子：
7.7
作者：
Barneda D;Planas-Iglesias J;Gaspar ML;Mohammadyani D;Prasannan S;Dormann D;Han GS;Jesch SA;Carman GM;Kagan V;Parker MG;Ktistakis NT;Klein-Seetharaman J;Dixon AM;Henry SA;Christian M
通讯作者：
Christian M