Temporal anomalies in immunological gene expression in a time series of wild mice: signature of an epidemic?
Temporal anomalies in immunological gene expression in a time series of wild mice: signature of an epidemic?
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DOI：
10.1371/journal.pone.0020070
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Jackson JA
中科院分区：
文献类型：
--
作者：
Friberg IM;Lowe A;Ralli C;Bradley JE;Jackson JA
虽然共同感染的生态重要性越来越多地认识到，在野生动物中的微生物病原体动力学分析通常集中在一个特设的子集的物种，由于检测技术的限制。在这里，我们展示了使用免疫基因（模式识别受体，细胞因子和转录因子）的表达谱作为一种手段，以确定，没有偏见，重要的急性微生物感染野生动物的可能性。使用在英国的木鼠种群作为模型，我们确定了显着的时间集群的个人极端表达的免疫介质在多个位点，典型的急性微生物感染。这些集群与夏季木鼠种群的人口扰动有关。动物在一个集群也有显着较高的个人大型寄生虫负担比同时代的“正常”的表达模式。如果观察到的极端转录谱是由感染因子诱导的，那么这意味着大型寄生虫可能参与介导个体对感染的易感性或恢复力。这里描述的调查形式，结合下一代核酸测序方法，用于在具有异常免疫转录谱的个体中广泛检测微生物感染因子，可能是揭示野生动物种群中循环的未识别的、生态上重要的感染因子的有力工具。
Although the ecological importance of coinfection is increasingly recognized, analyses of microbial pathogen dynamics in wildlife usually focus on an ad hoc subset of the species present due to technological limitations on detection. Here we demonstrate the use of expression profiles for immunological genes (pattern recognition receptors, cytokines and transcription factors) as a means to identify, without preconception, the likelihood of important acute microbial infections in wildlife. Using a wood mouse population in the UK as a model we identified significant temporal clusters of individuals with extreme expression of immunological mediators across multiple loci, typical of an acute microbial infection. These clusters were circumstantially associated with demographic perturbation in the summertime wood mouse population. Animals in one cluster also had significantly higher individual macroparasite burdens than contemporaries with “normal” expression patterns. If the extreme transcriptional profiles observed are induced by an infectious agent then this implicates macroparasites as a possible player in mediating individual susceptibility or resilience to infection. The form of survey described here, combined with next generation nucleic acids sequencing methods for the broad detection of microbial infectious agents in individuals with anomalous immunological transcriptional profiles, could be a powerful tool for revealing unrecognized, ecologically important infectious agents circulating in wildlife populations.
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影响因子：
5.8
作者：
Alzhanova D;Früh K
通讯作者：
Früh K
影响因子：
0.8
作者：
Kulldorff, M
通讯作者：
Kulldorff, M
影响因子：
64.8
作者：
Kash, John C.;Tumpey, Terrence M.;Katze, Michael G.
通讯作者：
Katze, Michael G.
DOI：
10.1016/j.bbrc.2005.08.209
发表时间：
2005-10-28
影响因子：
3.1
作者：
McKimmie, CS;Johnson, N;Fazakerley, JK
通讯作者：
Fazakerley, JK
DOI：
10.1111/j.1365-2656.2007.01302.x
发表时间：
2008-01
期刊：
The Journal of animal ecology
影响因子：
--
作者：
Burthe S;Telfer S;Begon M;Bennett M;Smith A;Lambin X
通讯作者：
Lambin X