Ethanol and dietary unsaturated fat (corn oil/linoleic acid enriched) cause intestinal inflammation and impaired intestinal barrier defense in mice chronically fed alcohol.
Ethanol and dietary unsaturated fat (corn oil/linoleic acid enriched) cause intestinal inflammation and impaired intestinal barrier defense in mice chronically fed alcohol.
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DOI：
10.1016/j.alcohol.2013.01.005
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发表时间：
2013-05
期刊：
影响因子：
2.3
通讯作者：
McClain, Craig J.
中科院分区：
文献类型：
--
作者：
Kirpich, Irina A.;Feng, Wenke;Wang, Yuhua;Liu, Yanlong;Beier, Juliane I.;Arteel, Gavin E.;Falkner, K. Cameron;Barve, Shirish S.;McClain, Craig J.
酒精和膳食脂肪都在酒精介导的多器官病理学中起重要作用，包括肠道和肝脏。在本研究中，我们假设酒精和膳食不饱和脂肪（USF）的组合会导致肠道炎症应激和粘液层改变，从而导致肠道屏障完整性的破坏。给C57 BL/6 N小鼠喂食含有EtOH并富含USF（玉米油/亚油酸）或SF（中链甘油三酯：牛脂）的Lieber-DeCarli液体饲料8周。肠组织学，形态计量学，炎症标志物，以及粘液保护因子的水平进行了评价。酒精和膳食USF引发肠道促炎反应，其特征在于TNF-α、MCP 1和MPO活性增加。此外，酒精和饮食USF，但不是SF，导致肠粘液层的改变，其特征在于回肠中Muc 2的表达减少。在抗菌因子Cramp的下调和TNF-α mRNA的增加之间观察到强相关性。因此，膳食不饱和脂肪（富含玉米油/LA）是啮齿动物中EtOH介导的肠道炎症反应和粘液层改变的重要促成因素。
Alcohol and dietary fat both play an important role in alcohol-mediated multi-organ pathology, including gut and liver. In the present study we hypothesized that the combination of alcohol and dietary unsaturated fat (USF) would result in intestinal inflammatory stress and mucus layer alterations, thus contributing to disruption of intestinal barrier integrity. C57BL/6N mice were fed Lieber-DeCarli liquid diets containing EtOH and enriched in USF (corn oil/linoleic acid) or SF (medium chain triglycerides: beef tallow) for 8 weeks. Intestinal histology, morphometry, markers of inflammation, as well as levels of mucus protective factors were evaluated. Alcohol and dietary USF triggered an intestinal pro-inflammatory response, characterized by increase in Tnf-α, MCP1, and MPO activity. Further, alcohol and dietary USF, but not SF, resulted in alterations of the intestinal mucus layer, characterized by decreased expression of Muc2 in the ileum. A strong correlation was observed between down-regulation of the antimicrobial factor Cramp and increased Tnf-α mRNA. Therefore, dietary unsaturated fat (corn oil/LA enriched) is a significant contributing factor to EtOH-mediated intestinal inflammatory response and mucus layer alterations in rodents.
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影响因子：
7.7
作者：
Cani, Patrice D.;Amar, Jacques;Burcelin, Remy
通讯作者：
Burcelin, Remy
DOI：
10.1152/ajpgi.2000.278.3.g467
发表时间：
2000-03-01
影响因子：
4.5
作者：
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通讯作者：
McGuckin MA
影响因子：
30.3
作者：
Lupp, Claudia;Robertson, Marilyn L.;Finlay, B. Brett
通讯作者：
Finlay, B. Brett